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品种反应性 主要应用 宿主 格式 抗体类型 Xn, H, Ht, M IHC, FC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3536 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Lamin B2 Antibody, clone LN43 背景信息 Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. 产品信息 格式 Purified 演示 Purified culture supernatant. Liquid in PBS containing 0.09% sodium azide. 应用 应用 This Anti-Lamin B2 Antibody, clone LN43 is validated for use in FC, WB, IH for the detection of Lamin B2. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
应用说明 Western blotting: 1:100-1:1,000; nuclear preparations recommended; detects 67-68kDa lamin B2 protein
Immunocytochemistry on fixed cells (methanol/acetone fixation): 1:100-1:200.Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Detergent insoluble fraction of potoroo cell line PtK1 克隆 LN43 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Lamin B2. Recognizes an epitope located in the C-terminal part of lamin B2. 同种型 IgG1 品种反应性 XenopusHumanHamsterMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_032737.2
Entrez基因汇总 The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. 基因符号 - LAMB2
- LMNB2
- Lamin-B2.
- LMN2
- MGC2721
UniProt编号 - Q03252
UniProt汇总 FUNCTION: SwissProt: Q03252 # Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
SIZE: 600 amino acids; 67689 Da
SUBCELLULAR LOCATION: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
PTM: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
DISEASE: SwissProt: Q03252 # Defects in LMNB2 are a cause of partial acquired lipodystrophy (APL) [MIM:608709]; also called Barraquer-Simons syndrome. APL is a rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidaemia, and complement deficiency. Mental retardation in some cases. APL is a sporadic disorder of unknown aetiology.
SIMILARITY: SwissProt: Q03252 ## Belongs to the intermediate filament family.
MISCELLANEOUS: The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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