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品种反应性 主要应用 宿主 格式 抗体类型 R, B, Gp, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3073 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-G Protein Goα Antibody, clone 2A 产品信息 格式 Purified 演示 Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide as a preservative. 应用 应用 Anti-G Protein Goα Antibody, clone 2A is an antibody against G Protein Goα for use in WB. 主要应用 - Western Blotting
应用说明 Western blot 1:3000.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Bovine brain Go-alpha purified by the method of Sternweis and Robishaw (J. Biol. Chem. [1984] 259, 13806). 克隆 2A 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Go-alpha. Strong reactivity with bovine (100%), human (70%), rat (61%) and mouse (54%). Slight reactivity with guinea pig (27%). Specificity confirmed by Western blot versus bovine G-alpha-t, rat Go-alpha, rat Gi-alpha-1, Gi-alpha-2, rat Gi-alpha-3, bovine Gs-alpha, and murine Go-alpha-A and Go-alpha-B. The carboxy-terminal splice variants of Go-alpha (Go-alpha-A and Go-alpha-B) expressed in bacterial lysates were compared for reactivity and found to be equal . As Go-alpha-A and Go-alpha-B have >95% homology to rat Go-alpha-1 and human Go-alpha-2, MAB3073 will detect these G-proteins also. 同种型 IgG1 品种反应性 RatBovineGuinea PigHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_138736.1
- NM_020988.1
基因符号 - GNAO
- GNAO1
- DKFZp686O0962
- G-ALPHA-o
UniProt编号 - P29777
UniProt汇总 FUNCTION: SwissProt: P29777 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear.| P09471 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear.
SIZE: 354 amino acids; 40087 Da
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
DOMAIN: SwissProt: P29777
SIMILARITY: SwissProt: P29777 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily. | P09471 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for upt to 6 months after date of receipt.
During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.包装信息 数量 100 µg
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