merck millipore,默克密理博,71086,KOD Hot Start DNA Polymerase

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merck millipore,默克密理博,71086,KOD Hot Start DNA Polymerase
产品名称：KOD Hot Start DNA Polymerase
产品型号：71086
Heat-activatable form of KOD DNA Polymerase for increased specificity and convenient PCR setup

merck millipore,默克密理博,71086,KOD Hot Start DNA Polymerase

产品介绍

merck millipore,默克密理博,71086,KOD Hot Start DNA Polymerase

描述

概述

KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.

Source	Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
Concentration	1.0 U/µl
Nicking activity	None detected
Amplification effiency	Functional PCR; inhibition of activity at 21°C verified
Storage	–20°C

*Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

产品目录编号

71086

品牌系列

Novagen®

特点及优点

Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
Amplifies genomic DNA templates up to 12 kbp
Amplifies plasmid and lambda DNA templates up to 21 kbp
Successfully amplifies GC-rich sequences
Eliminates mispriming and primer-dimer formation
Convenient room-temperature setup compatible with automation
Optimal KOD Hot Start Buffer for PCR performance over a wide range of targets

参考
参考	Mizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.

产品信息
部件
声明	Manufactured by Toyobo and distributed by EMD. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

产品信息

部件

声明

Manufactured by Toyobo and distributed by EMD. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.