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merck millipore,默克密理博,MAB3328,Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8
产品名称：Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8
产品型号：MAB3328
Detect MMP-14 using this Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8 validated for use in ELISA, IP & WB.
merck millipore,默克密理博,MAB3328,Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB3328 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8 Alternate Names - MT1-MMP
背景信息 MT1-MMP plays an important role during endothelial cell migration and matrix remodeling. Although the role of MT1-MMP in endothelial cell motility is not fully characterized, its activity appears to modulate endothelial migration, invasion, and formation of capillary tubes during the angiogenic response (Galvez, 2001). Mt1-MMP also appears to play a key role in monocyte revruitment during inflammation (Salomon, 2005). 产品信息 格式 Purified 演示 Purified immunoglobulin by Protein A chromatography . Liquid in 0.2M phosphate, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide. 应用 应用 Detect MMP-14 using this Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8 validated for use in ELISA, IP & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western Blot
Immunohistochemistry: Frozen and paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Blocking: 10-15μg/mL
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide: amino acid sequence 218-233 within the catalytic domain 表位 catalytic domain 克隆 LEM-2/15.8 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 LEM-2/15.8 reacts with human MT1-MMP and displays crossreactivity with mouse specimens. This antibody was generated against the catalytic domain of MT1-MMP and is able to inhibit enzyme activity. 同种型 IgG1κ 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004995.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. 基因符号 - EC 3.4.24.80
- MMP14
- MTMMP1
- MMP-14
- MT1MMP
- MT1-MMP
- MMP-X1
UniProt编号 - P50281
UniProt汇总 FUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2° to 8°C for up to 12 months from date of receipt. 包装信息 数量 100 µg
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