merck millipore,默克密理博,ABE171,Anti-MeCP2 Antibody

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merck millipore,默克密理博,ABE171,Anti-MeCP2 Antibody
产品名称：Anti-MeCP2 Antibody
产品型号：ABE171
Anti-MeCP2 Antibody is a Chicken Polyclonal Antibody for detection of MeCP2 also known as methyl CpG binding protein 2 (Rett syndrome), mental retardation X-linked 16 & has been validated in WB.

merck millipore,默克密理博,ABE171,Anti-MeCP2 Antibody

产品介绍

merck millipore,默克密理博,ABE171,Anti-MeCP2 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H, M, R	ChIP, WB	Ch	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABE171
描述	Anti-MeCP2 Antibody
Alternate Names	methyl-CpG-binding protein 2 methyl CpG binding protein 2 (Rett syndrome) mental retardation, X-linked 16 MeCp-2 protein mental retardation, X-linked 79
背景信息	MeCp2 (methyl-CpG-binding protein 2) binds methylated CpG sequences in double-stranded DNA resulting in the suppression of transcription. Although MeCp2 is not essential in stem cells, it is critical for embryonic development. This protein seems to be particularly important for mature nerve cells, where it is present in high levels. The MeCp2 protein is likely to be involved in turning off ("repressing" or "silencing") several other genes. Consistent with this role, mutations of MeCp2 are linked to Rett syndrome (RTT), a progressive neurological disorder that is one of the most common causes of mental retardation in females.

产品信息
格式	Affinity Purified
控制	SH-SY5Y nuclear extract
演示	Purified chicken polyclonal in buffer with 0.05% sodium azide and 50% glycerol.

应用
应用	Anti-MeCP2 Antibody is a Chicken Polyclonal Antibody for detection of MeCP2 also known as methyl CpG binding protein 2 (Rett syndrome), mental retardation X-linked 16 & has been validated in WB.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory in ChIP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human MeCp2.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Chicken
同种型	IgY
品种反应性	HumanMouseRat
Species Reactivity Note	Demonstrated to react with Human, Mouse, and Rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_004983
Entrez基因汇总	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq].
基因符号	MRX16 MECP2 AUTSX3 RS MRXS13 MRXSL RTT PPMX RTS MRX79 MeCp2
纯化方法	Antigen Affinity Purified
UniProt编号	P51608
UniProt汇总	FUNCTION: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. SIZE: 486 amino acids; 52441 Da SUBUNIT: Interacts with FNBP3 (By similarity). SUBCELLULAR LOCATION: Nucleus. Note=Colocalized with methyl-CpG in the genome. TISSUE SPECIFICITY: Present in all adult somatic tissues tested. PTM: Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation (By similarity). DISEASE: Defects in MECP2 may be a cause of Angelman syndrome (AS) [MIM:105830]; also known as happy puppet syndrome. AS is a neurodevelopmental disorder characterized by severe mental retardation, absent speech, ataxia, sociable affect and dysmorphic facial features. AS and Rett syndrome have overlapping clinical features. & Defects in MECP2 are the cause of mental retardation syndromic X-linked type 13 (MRXS13) [MIM:300055]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXS13 patients manifest mental retardation associated with other variable features such as spasticity, episodes of manic depressive psychosis, increased tone and macroorchidism. & Defects in MECP2 are the cause of Rett syndrome (RTT) [MIM:312750]. RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements and develop microcephaly, seizures, autism, ataxia, intermittent hyperventilation, and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood. & Defects in MECP2 may be the cause of susceptibility to X- linked autism 3 (AUTSX3) [MIM:300496]. AUTSX3 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. & Defects in MECP2 may be a cause of nonprogressive encephalopathy with neonatal onset [MIM:300005]. & A chromosomal duplication involving MECP2 is the cause of mental retardation syndromic X-linked Lubs type (MRXSL) [MIM:300260]. Increased dosage of MECP2 appears to be responsible for the mental retardation phenotype. The main features present in affected males are severe to profound mental retardation with onset at birth, axial and facial hypotonia, progressive spasticity predominantly at the lower limbs, seizures and recurrent infections. SIMILARITY: Contains 2 A.T hook DNA-binding domains. & Contains 1 MBD (methyl-CpG-binding) domain.

产品使用声明
质量保证	Evaluated by Western Blot in SH-SY5Y nuclear extract. Western Blot Analysis: 1 µg/mL of this antibody detected MeCp2 on 10 µg of SH-SY5Y nuclear extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.