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品种反应性 主要应用 宿主 格式 抗体类型 Sh, B, Ca, H, M, Po, R, Rb WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB326R 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CNPase Antibody, clone 11-5B 背景信息 The enzyme 2', 3'-cyclic nucleotide 3'-phosphodi-esterase (CNP) is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system (Sprinkle, 1989). By virtue of this cell-specific expression, CNP is recognized as a characteristic marker for these two myelin-producing glial cell types (Sprinkle, 1989; Kim et al., 1984; Sheedlo & Sprinkle, 1984; McMorris et al., 1984). Beyond its enzymatic activity of cleaving the 2', 3'-cyclic terminus of nucleotides (Sprinkle, 1989), the physiological role of CNP is still under investigation. CNP activity has been correlated with myelin and myelin formation, and a dramatic decrease in CNP activity is associated with demyelinating diseases such as multiple sclerosis (Sprinkle, 1989). This enzyme is composed of two proteins, CNP1 (46 kD) and CNP2 (48 kD) (Sprinkle, 1989; Sprinkle et al., 1987). Although the ratio of CNP1/CNP2 may vary from species to species, their shared primary sequence is conserved phyIogenetically. CNP has recently been localized to human chromosome 17 by amplification of somatic cell hybrid DNA using the polymerase chain reaction (PCR) and by Southern blotting of Hind Ill genomic DNA digests (Sprinkle et al., 1991). Since anti-CNP reacts with a highly conserved region of the enzyme, it can be considered as pan-anti-CNP (Sprinkle et al., 1987). Anti-CNP can be used as a marker to identify Schwann cells and oligodendrocytes in cell cuIture and in tissue sections, as well as to localize CNP in cell membrane fractions. As CNP is expressed relatively early in postnatal development, anti-CNP is especially useful for the early identification of oligodendrocytes. 产品信息 格式 Purified 控制 - Oligodendrocyte culture, Brain lysate
演示 Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, pH 7.6, 0.25M NaCl with 0.1% sodium azide. 应用 应用 This Anti-CNPase Antibody, clone 11-5B is validated for use in IC, IH, IH(P), WB for the detection of CNPase. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
A previous lot of this antibody was used in IH (10 μg/mL antibody, prepared fresh daily).
Immunocytochemistry:
A previous lot of this antibody was used in IC (10 μg/mL antibody, prepared fresh daily).
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified human 2’, 3’-cyclic nucleotide 3’-phosphodiesterase 克隆 11-5B 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Anti-CNP reacts with both CNP1 and CNP2 in many species. 同种型 IgG1 品种反应性 SheepBovineCanineHumanMousePigRatRabbit 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_033133.4
基因符号 - EC 3.1.4.37
- CNP
- CNPase
- CNP1
纯化方法 Protein A purfied UniProt编号 - P09543
UniProt汇总 SIZE: 421 amino acids; 47579 Da
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.产品使用声明 质量保证 Evaluated by Western Blot on Mouse brain lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected CNPASE 1/2 on 10 µg of Mouse brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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