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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB Rb Serum Polyclonal Antibody 描述 产品目录编号 06-1295 描述 Anti-CDT1 Antibody Alternate Names - chromatin licensing and DNA replication factor 1
- DNA replication factor
- Double parked homolog
- Double parked, Drosophila, homolog of
背景信息 CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006). 产品信息 格式 Serum 控制 - HeLa cell lysate
演示 Rabbit polyclonal serum with 0.05% sodium azide. 应用 应用 Use Anti-CDT1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect CDT1 also known as DNA replication factor, Double parked homolog. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human CDT1. 宿主 Rabbit 特异性 This antibody recognizes CDT1. 品种反应性 MouseHumanRat Species Reactivity Note Proven to react with human, mouse and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005905.1
基因符号 - RIS2
- DUP
纯化方法 Unpurified UniProt编号 - Q9H211
UniProt汇总 FUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity.
SUBCELLULAR LOCATION: Nucleus.
DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
PTM : Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination.
Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.
SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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