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品种反应性 主要应用 宿主 格式 抗体类型 R IHC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB5168-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CHAPSYN-110 Antibody Alternate Names - PSD-93, Discs Large Homolog 2, DLG2
- PSD-93
- Discs Large Homolog 2
产品信息 格式 Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 10 ng of protein per lane. For negative control, preincubate 1 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 This Anti-CHAPSYN-110 Antibody is validated for use in WB, IH for the detection of CHAPSYN-110. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blot: 1 to 3 μg/mL (1:300-1:1000) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GST fusion protein (Smith & Johnson 1988) and of rat chapsyn-110 (amino acids 336-379) (Brenman et al. 1996; Kim et al. 1996). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes a full length chapsyn-110 protein. It has exhibited no cross reactivity with other known related proteins tested so far. 品种反应性 Rat Species Reactivity Note The immunogen sequence is 42 of 44 amino acids identical in human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001364.2
Entrez基因汇总 This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Alternatively spliced transcript variants encoding distinct isoforms have been described but their full-length nature has yet to be completely determined. 基因符号 - DKFZp781D1854
- DLG2
- PSD-93
- DKFZp781E0954
- Chapsyn-110
- FLJ37266
- MGC131811
- chapsyn-110
UniProt编号 - Q15700
UniProt汇总 FUNCTION: SwissProt: Q15700 # Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses (By similarity).
SIZE: 870 amino acids; 97542 Da
SUBUNIT: Interacts with NOS1/nNOS through second PDZ domain (By similarity). Interacts with C-terminus of KCNJ2/Kir2.1 through one of its PDZ domains.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Concentrated in soma and postsynaptic density of a subset of neurons (By similarity).
DOMAIN:SwissProt: Q15700 Isoform 2 has an L27 domain close to N-terminus.
PTM: Palmitoylation of isoform 1 is not required for targeting to postsynaptic density (By similarity).
SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 3 PDZ (DHR) domains. & Contains 1 SH3 domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 200 µL
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