- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB1976H,Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated
产品名称：Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated
产品型号：MAB1976H
Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated detects level of Integrin αVβ3 & has been published & validated for use in FC, IH.
merck millipore,默克密理博,MAB1976H,Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, Av, B, Ca, Ch, H, Mk, Po IHC, FC M Phycoerythrin Monoclonal Antibody 描述 产品目录编号 MAB1976H 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated 概述 EMISSION MAXIMUM:
580 nmAlternate Names - CD51/CD61
- Vitronectin Receptor
背景信息 The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors. 产品信息 格式 Phycoerythrin 控制 - POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995).
NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV; Chen, 1995).
演示 Liquid in 0.01M sodium phosphate (pH7.6), 0.25 M NaCl, 0.05% sodium azide, 0.01% Thimerosal and 15 mg/mL BSA added as a stabilizer. 应用 应用 Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated detects level of Integrin αVβ3 & has been published & validated for use in FC, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Flow Cytometry: 1-3 μg LM609 per 0.5 x 10e6 cells in 100μL (Smith, 1996).
Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.
Optimal working dilutions and protocols must be determined by end user.生物信息 免疫原品种 Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987). 克隆 LM609 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Monoclonal antibody LM609 is reactive with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
ABSORPTION MAXIMA: 90 nm, 545 nm and 565 nm同种型 IgG1 品种反应性 RabbitAvianBovineCanineChickenHumanMonkeyPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002210.2
Entrez基因汇总 ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. 基因符号 - VNRA
- ITGAV
- DKFZp686A08142
- MSK8
- CD51
非反应性品种 - Rat
- Mouse
UniProt编号 - P06756
UniProt汇总 FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months. Protect from Light. Do not store as a dilute solution. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,07-771,Anti-acetyl-Histone H2A.Z (Lys10) Antibody,07-771
下一件merck millipore产品：merck millipore,默克密理博,238811,Cdk9 Inhibitor II - CAS 140651-18-9 - Calbiochem
相关产品
merck millipore,默克密理博,MABE866,Anti-Mad2 Antibody, clone AS55-A12
merck millipore,默克密理博,AB9402,Anti-Serotonin Receptor 1F Antibody
merck millipore,默克密理博,AB1929,Anti-Integrin β1 Antibody, phospho-specific [Thr788/Thr789]
merck millipore,默克密理博,05-588,Anti-HET/SAF-B Antibody, clone 6F7
merck millipore,默克密理博,07-771,Anti-acetyl-Histone H2A.Z (Lys10) Antibody,07-771
merck millipore,默克密理博,238811,Cdk9 Inhibitor II - CAS 140651-18-9 - Calbiochem
merck millipore,默克密理博,14-492,GSK3α Protein, active, 10 µg
merck millipore,默克密理博,PC679,Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb
merck millipore,默克密理博,616390,Toxin II, Anemonia sulcata - CAS 60748-45-0 - Calbiochem
merck millipore,默克密理博,04-921,Anti-GluR6/7 Antibody, clone NL9, rabbit monoclonal