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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-771 描述 Anti-acetyl-Histone H2A.Z (Lys10) Antibody | 07-771 Alternate Names - Htz1
- H2A histone family, member Z
- H2AZ histone
- histone H2A.Z
背景信息 H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control. 产品信息 格式 Affinity Purified 控制 - yeast whole cell extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 主要应用 - Western Blotting
应用说明 Western Blot (Snap i.d.) Analysis: 4 µg/mL from a representative lot detected Histone H2A.Z in 10 µg of yeast whole cell extract. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to yeast Histone H2A.Z when acetylated at Lys10. 表位 Histone H2A.Z acetylated Lys10 浓缩 0.5 mg/mL 宿主 Rabbit 特异性 This antibody recognizes Histone H2A.Z when acetylated at Lys10. 品种反应性 Human Species Reactivity Note Demonstrated to react with yeast. Predicted to react with Kluyveromyces lactis based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_014631
基因符号 - H2AFZ
- H2A.z
- H2A/z
- H2AZ
修改 - Acetylation
纯化方法 Affinity Purified UniProt编号 - Q12692
UniProt汇总 FUNCTION: Variant histone H2A which can replace H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. This variant is enriched at promoters, it may keep them in a repressed state until the appropriate activation signal is received. Near telomeres, it may counteract gene silencing caused by the spread of heterochromatin proteins. Required for the RNA polymerase II and SPT15/TBP recruitment to the target genes. Involved in chromosome stability.
SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2A.Z forms an heterodimer with H2B. H2A.Z associates with the VPS72/SWC2 subunit of the SWR1 chromatin remodeling complex. Interacts also with RBP1/DNA-directed RNA polymerase II largest subunit. Interacts with NAP1.
SUBCELLULAR LOCATION: Nucleus
PTM: Acetylated by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex, and/or by GCN5, a component of the SAGA complex, to form H2A.ZK3Ac, H2A.ZK8Ac, H2A.ZK10Ac and H2A.ZK14Ac once deposited into chromatin. Acetylation is required for function at telomeres. H2A.ZK14Ac is acetylated at the promoters of active genes.
MISCELLANEOUS: Present with 2840 molecules/cell in log phase SD medium.
In contrast to H2A1 and H2A2, appears to be weakly or not sumoylated.
SEQUENCE SIMILARITIES: Belongs to the histone H2A family.
CAUTION: To ensure consistency between histone entries, we follow the 'Brno' nomenclature for histone modifications, with positions refering to those used in the literature for the 'closest' model organism. Due to slight variations in histone sequences between organisms and to the presence of initiator methionine in UniProtKB/Swiss-Prot sequences, the actual positions of modified amino acids in the sequence generally differ. In this entry the following conventions are used: H2A.ZK3ac = acetylated Lys-4; H2A.ZK8ac = acetylated Lys-9; H2A.ZK10ac = acetylated Lys-11; H2A.ZK14ac = acetylated Lys-15.产品使用声明 质量保证 Evaluated by Western Blot in yeast whole cell extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H2A.Z in 10 µg of yeast whole cell extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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