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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS90 描述 Anti-Notch 1 Antibody, extracellular Alternate Names - neurogenic locus notch homolog protein 1
- Notch homolog 1, translocation-associated (Drosophila)
- Notch (Drosophila) homolog 1 (translocation-associated)
- Translocation-associated notch protein TAN-1
背景信息 The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm). 产品信息 格式 Affinity Purified 控制 - Human lymph node lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Notch 1 Antibody, extracellular detects level of Notch 1 & has been published & validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: 1:400 dilution of this antibody has been shown to detect Notch 1 in gastrointestinal and colorectal carcinoma tissue. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the extracellular domain of rat Notch 1. 表位 Extracellular domain 宿主 Rabbit 特异性 This antibody recognizes Notch 1. 品种反应性 RatHuman Species Reactivity Note Demonstrated to react with human and rat. Expected to react with mouse (97% sequence homology), chimpanzee (92% sequence homology) and canine (91% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001099191
Entrez基因汇总 Transmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD]. 基因符号 - Notch 1
- TAN1
- notch1
- hN1
纯化方法 Affinity Purfied UniProt编号 - Q07008
UniProt汇总 FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity). Acts instructively to control the cell fate determination of CNS multipotent progenitor cells, resulting in astroglial induction and neuron/oligodendrocyte suppression.
SUBUNIT STRUCTURE: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity).
Notch 1 intracellular domain: Nucleus (By similarity). Note: Following proteolytical processing NICD is translocated to the nucleus (By similarity).
TISSUE SPECIFICITY: Expressed in the brain, kidney and spleen. Expressed in postnatal central nervous system (CNS) germinal zones and, in early postnatal life, within numerous cells throughout the CNS. Found in both subventricular and ventricular germinal zones.
DEVELOPMENTAL STAGE: In the embryo, highest levels occur between days 12 and 14 and decrease rapidly to much lower levels in the adult.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).
Phosphorylated (By similarity).
O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose (By similarity).
SEQUENCE SIMILARITIES: Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 36 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.产品使用声明 质量保证 Evaluated by Western Blot in human lymph node lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected Notch 1 on 10 µg of human lymph node lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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