merck millipore,默克密理愽
  • merck millipore,默克密理博,04-574,Anti-Caspase-8 (NT) Antibody, rabbit monoclonal

  • merck millipore,默克密理博,04-574,Anti-Caspase-8 (NT) Antibody, rabbit monoclonal

    产品名称：Anti-Caspase-8 (NT) Antibody, rabbit monoclonal
    产品型号：04-574
    Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Caspase-8 (N-Term) Antibody is validated f More>>

    merck millipore,默克密理博,04-574,Anti-Caspase-8 (NT) Antibody, rabbit monoclonal

  • 产品介绍
  • merck millipore,默克密理博,04-574,Anti-Caspase-8 (NT) Antibody, rabbit monoclonal

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HFC, WBRbPurifiedMonoclonal Antibody
    描述
    产品目录编号 04-574
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Caspase-8 (NT) Antibody, rabbit monoclonal
    背景信息 Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
    产品信息
    格式 Purified
    演示 Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
    应用
    应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Caspase-8 (N-Term) Antibody is validated for use in IP, WB, IH(P), FC for the detection of Caspase-8 (N-Term).
    主要应用
    • Flow Cytometry
    • Western Blotting
    应用说明 WB: 1:3000
    IHC: 1:100 (Paraffin-embedded human breast adenocarcinoma)
    Flow Cytometry: 1:50
    IP: 1:50
    生物信息
    免疫原品种 A synthetic peptide corresponding to N-terminal residues of human Caspase-8. The antibody should recognize all splice isoforms of Caspase-8.
    克隆 E6
    宿主 Rabbit
    特异性 Recognizes Human Caspase-8 (N-Term).
    品种反应性 Human
    Species Reactivity Note Human (positive control: Jurkat cell lysate)
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_033358.3
    • NM_001080124.1
    • NM_001080125.1
    • NM_001228.4
    • NM_033355.3
    • NM_033356.3
    Entrez基因汇总 This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
    基因符号
    • CAP4
    • CASP8
    • MCH5
    • MGC78473
    • CASP-8
    • MACH
    • ALPS2B
    • FLICE
    • procaspase-8
    UniProt编号
    • Q14790
    UniProt汇总 FUNCTION: SwissProt: Q14790 # Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoforms 5, 6, 7 and 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
    SIZE: 479 amino acids; 55391 Da
    SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1.
    SUBCELLULAR LOCATION: Cytoplasm.
    TISSUE SPECIFICITY: Isoforms 1, 5 and 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus, and liver. Barely detectable in brain, testis, and skeletal muscle.DOMAIN:SwissProt: Q14790 Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
    PTM: Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. & Phosphorylated upon DNA damage, probably by ATM or ATR.
    DISEASE: SwissProt: Q14790 # Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
    SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store at -20 °C. Stable for 12 months from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,04-574,Anti-Caspase-8 (NT) Antibody, rabbit monoclonal

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