- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,HTS095M,ChemiScreen™ Membrane Preparation Recombinant Human Kappa Opioid Receptor
产品名称：ChemiScreen™ Membrane Preparation Recombinant Human Kappa Opioid Receptor
产品型号：HTS095M
Human Kappa / OP2 / KOP / KOR GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS095M,ChemiScreen™ Membrane Preparation Recombinant Human Kappa Opioid Receptor
- 产品介绍
- merck millipore,默克密理博,HTS095M,ChemiScreen™ Membrane Preparation Recombinant Human Kappa Opioid Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 Kappa / OP2 / KOP / KOR 描述 产品目录编号 HTS095M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Membrane Preparation
Recombinant Human Kappa Opioid Receptor概述 Human OPK1, κ 背景信息 Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, µ, κ, and δ, that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the µ opioid receptor. Activation of the κ opioid receptor by selective agonists also produces analgesia, primarily mediated by spinal sites, but causes dysphoria and psychosis instead of euphoria. The κ receptor at central and peripheral sites is also largely responsible for the anti-diarrheal effects of opiates (Dhawan et al., 1996). Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of κ interactions with diprenorphine. The membrane preparations exhibit a Kd of 2.3-3.7 nM for [3H]-diprenorphine. With 10 µg/well κ Membrane Prep and 2.5 nM [3H]-diprenorphine, a greater than 3-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H-labeled diprenorphine at 2.5 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human Kappa / OP2 / KOP / KOR GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_000912.3
基因符号 - KOR
- OPRK1
- OPRK
- KOR-1
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 Kappa / OP2 / KOP / KOR 靶子系列 Opioid UniProt编号 - P41145
UniProt汇总 FUNCTION: SwissProt: P41145 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
SIZE: 380 amino acids; 42645 Da
SUBUNIT: Binds SLC9A3R1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P41145 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with Binding Buffer. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Binding Buffer. The plate is dried and counted.
Binding buffer: 50 mM Tris-HCl pH 7.4, filtered and stored at 4°C
Radioligand: [3H] diprenorphine (Perkin Elmer # NET1121)
产品使用声明 质量保证 SPECIFICATIONS:
Signal:background and specific binding values obtained in a competition binding assay with κ membrane prep:10 µg/well Signal:Background 4.47 Specific Binding (cpm) 939
1 unit = 10 µg membrane preparation
Bmax: 21.73 pmol/mg
Kd: 3.0 nM
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,317700,Dkk-1 Inhibitor, WAY-262611, HCl - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells
相关产品
merck millipore,默克密理博,189497,Autophagy Inducer, STF-62247 - CAS 315702-99-9 - Calbiochem
merck millipore,默克密理博,SF-900,miR-320b; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,ABS90,Anti-Notch 1 Antibody, extracellular
merck millipore,默克密理博,362314,Glycosyltransferase Affinity Gel-UDP
merck millipore,默克密理博,317700,Dkk-1 Inhibitor, WAY-262611, HCl - Calbiochem
merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells
merck millipore,默克密理博,371715,Gliotoxin, Gladiocladium fimbriatum - CAS 67-99-2 - Calbiochem
merck millipore,默克密理博,370654,PKG Inhibitor - Calbiochem
merck millipore,默克密理博,MAPHN0B50,MultiScreen-PH，0.65 µm，不透明，非无菌