merck millipore,默克密理愽
  • merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells

  • merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells

    产品名称：RosettaBlue™(DE3)pLysS Competent Cells
    产品型号：71034
    RosettaBlue host strains combine high transformation efficiency with enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

    merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells

  • 产品介绍
  • merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells

    描述
    概述 RosettaBlue™ host strains are NovaBlue derivatives that combine high transformation efficiency and recA endA lacIq mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In RosettaBlue(DE3)pLysS, the rare tRNA genes are present on the same plasmid that carries the T7 lysozyme gene. Blue/white screening is not possible with RosettaBlue(DE3) strains due to the presence of the lacZ α-peptide coding sequence in the λDE3 prophage.

    DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

    pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
    Genotype: endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac (DE3) F′[proA+B+ lacIqZΔM15::Tn10] LysSRARE (CamR, TetR)
    产品目录编号 71034
    品牌系列 Novagen®
    产品信息
    部件
    保证高效 Guaranteed efficiency: > 1 x 10⁶ cfu/µg
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Standard Handling
    储存 ≤ -70°C
    无需冷冻 No
    布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

    merck millipore,默克密理博,71034,RosettaBlue™(DE3)pLysS Competent Cells

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下一件merck millipore产品：merck millipore,默克密理博,371715,Gliotoxin, Gladiocladium fimbriatum - CAS 67-99-2 - Calbiochem


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