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品种反应性 主要应用 宿主 格式 抗体类型 R, H IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3274-50UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aquaporin 2 Antibody Alternate Names - WCD-CD
- AQP2
- AQP-CD
产品信息 格式 Affinity Purified 控制 - Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). 应用 应用 Anti-Aquaporin 2 Antibody detects level of Aquaporin 2 & has been published & validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blotting: 1.0-3.0 μg/mL (1:200-1:600 using ECL) on rat kidney membranes.
Immunohistochemistry
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Highly purified peptide corresponding to residues 254-271 of rat or mouse Aquaporin 21,2 with an additional N-terminal lysine and tyrosine. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Aquaporin 2.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 2 is identical in rat and mouse and only slightly different in human (17/18 amino acids). Reactivity with other species has not yet been tested.品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000486.3
Entrez基因汇总 This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus. 基因符号 - WCH-CD
- AQP2
- AQP-CD
- aquaporin-CD
- Aquaporin-2
- Aquaporin-CD
- AQP-2
- MGC34501
UniProt编号 - P41181
UniProt汇总 FUNCTION: SwissProt: P41181 # Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
SIZE: 271 amino acids; 28837 Da
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane.
TISSUE SPECIFICITY: Expressed in renal collecting tubules.
DOMAIN: SwissProt: P41181 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
PTM: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
DISEASE:SwissProt: P41181 # Defects in AQP2 are a cause of autosomal nephrogenic diabetes insipidus, type I (AD-NDI) [MIM:125800]. It is characterized by excessive water drinking (polydypsia) and urine excretion (polyuria).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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