- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB1951Z,Anti-Integrin β1 Antibody, clone P4G11, azide free
产品名称：Anti-Integrin β1 Antibody, clone P4G11, azide free
产品型号：MAB1951Z
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.
merck millipore,默克密理博,MAB1951Z,Anti-Integrin β1 Antibody, clone P4G11, azide free
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, H, Mk IHC, ELISA, FC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1951Z 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin β1 Antibody, clone P4G11, azide free Alternate Names - MAB1951
- CD29
产品信息 格式 Purified 演示 Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives. 应用 应用 This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1. 主要应用 - Immunohistochemistry
- ELISA
- Flow Cytometry
- Immunocytochemistry
应用说明 Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human IL-1beta activated endothelial cells 克隆 P4G11 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent. 同种型 IgG1 品种反应性 RabbitHumanMonkey 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_133376.1
- NM_002211.2
- NM_033666.1
- NM_033667.1
- NM_033668.1
- NM_033669.1
Entrez基因汇总 Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. 基因符号 - MSK12
- ITGB1
- GPIIA
- OTTHUMP00000063731
- OTTHUMP00000046253
- OTTHUMP00000063733
- VLAB
- OTTHUMP00000063732
- MDF2
- FNRB
- CD29
UniProt编号 - P05556
UniProt汇总 FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 798 amino acids; 88465 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.
TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).
SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,12-408,Histone H2B Peptide, biotin conjugate, residues 21-41
下一件merck millipore产品：merck millipore,默克密理博,MABT75,Anti-Titin (NT/Z-domain) Antibody, clone 17D11.1
相关产品
merck millipore,默克密理博,444896,MAS 7 - CAS 145854-59-7 - Calbiochem
merck millipore,默克密理博,386040,Anti-Hsp90 Mouse mAb (AC88)
merck millipore,默克密理博,AB15048,Anti-CNT2 Antibody
merck millipore,默克密理博,TCP1-F84,Temperature Calibration Plate
merck millipore,默克密理博,12-408,Histone H2B Peptide, biotin conjugate, residues 21-41
merck millipore,默克密理博,MABT75,Anti-Titin (NT/Z-domain) Antibody, clone 17D11.1
merck millipore,默克密理博,328006,ERK Inhibitor - CAS 1049738-54-6 - Calbiochem
merck millipore,默克密理博,SF-145,Beta Actin; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,AB3274-50UL,Anti-Aquaporin 2 Antibody
merck millipore,默克密理博,04-1008,Anti-NF-κ-B p65 Antibody, clone EP2161Y, rabbit monoclonal