• merck millipore,默克密理博,178612,Anti-Aquaporin 2 (254-271) Rabbit pAb
  • merck millipore,默克密理博,178612,Anti-Aquaporin 2 (254-271) Rabbit pAb

    产品名称:Anti-Aquaporin 2 (254-271) Rabbit pAb
    产品型号:178612
    This Anti-Aquaporin 2 (254-271) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Aquaporin 2 (254-271).

    merck millipore,默克密理博,178612,Anti-Aquaporin 2 (254-271) Rabbit pAb

  • 产品介绍
  • merck millipore,默克密理博,178612,Anti-Aquaporin 2 (254-271) Rabbit pAb

    重要规格表

    宿主
    Rb
    描述
    概述 Recognizes the ~40 kDa and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide (AQP2254-271 immunogen peptide).
    产品目录编号 178612
    品牌系列 Calbiochem®
    同义词 Anti-AQP2, Anti-AQP-CD, Anti-WCH-CD
    应用数据
    Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence.

    Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
    参考
    参考 Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
    Fushimi, K., et al. 1993. Nature 361, 549.
    Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
    Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
    产品信息
    形式 Lyophilized
    配方 50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
    防腐剂 ≤0.1% sodium azide
    应用
    应用参考 Immunoprecipitation Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Immunohistochemistry Fushimi, K., et al. 1993. Nature 361, 549. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
    主要应用 Immunoblotting (Western Blotting)
    Immunohistochemistry
    Immunoprecipitation
    生物信息
    免疫原 a synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
    免疫原品种 Mouse/Rat
    宿主 Rabbit
    同种型 IgG
    品种 human, mouse, rat
    浓缩标签 Please refer to vial label for lot-specific concentration
    储存和货运信息
    货运代码 Ambient Temperature Only
    毒性 Irritant
    储存 -20°C
    无需冷冻 No
    特殊指南 Please see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).

    merck millipore,默克密理博,178612,Anti-Aquaporin 2 (254-271) Rabbit pAb

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