merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB2030,Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

  • merck millipore,默克密理博,MAB2030,Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

    产品名称：Anti-Chondroitin 4 Sulfate Antibody, clone BE-123
    产品型号：MAB2030
    Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.

    merck millipore,默克密理博,MAB2030,Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

  • 产品介绍
  • merck millipore,默克密理博,MAB2030,Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Sh, B, Ca, H, M, RWB, ELISAMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB2030
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Chondroitin 4 Sulfate Antibody, clone BE-123
    产品信息
    格式 Ascites
    演示 Ascites fluid containing no preservatives.
    应用
    应用 Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
    主要应用
    • Western Blotting
    • ELISA
    应用说明 ELISA at 1:400-1:3,200.

    RIA at 1:50-1:6,400.

    Immunohistochemistry

    Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
    In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
    Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Mouse proteoglycan.
    克隆 BE-123
    宿主 Mouse
    特异性 Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.
    同种型 IgG1κ
    品种反应性 SheepBovineCanineHumanMouseRat
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_001897.4
    Entrez基因汇总 A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
    基因符号
    • MCSPG
    • MEL-CSPG
    • HMW-MAA
    • CSPG4
    • MSK16
    • MCSP
    • NG2
    UniProt编号
    • Q6UVK1
    UniProt汇总 FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
    SIZE: 2322 amino acids; 250497 Da
    SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
    SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
    TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
    PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
    SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
    MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB2030,Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

上一件merck millipore产品：merck millipore,默克密理博,07-1841,Anti-acetyl-Tip60 Antibody (Lys523)
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