merck millipore,默克密理博,ECM221,QCM Laminin Migration Assay (24-well, fluorometric)

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merck millipore,默克密理博,ECM221,QCM Laminin Migration Assay (24-well, fluorometric)
产品名称：QCM Laminin Migration Assay (24-well, fluorometric)
产品型号：ECM221
This QCM Laminin Migration Assay incorporates a 5 µm porous membrane Boyden chamber & mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependen More>>

merck millipore,默克密理博,ECM221,QCM Laminin Migration Assay (24-well, fluorometric)

产品介绍

merck millipore,默克密理博,ECM221,QCM Laminin Migration Assay (24-well, fluorometric)

重要规格表

检测方法
Fluorescent

描述
产品目录编号	ECM221
商名	Chemicon QCM
描述	QCM Laminin Migration Assay (24-well, fluorometric)
概述	Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Introduction Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Cell migration may be evaluated using several different methods; the most widely accepted being the Boyden Chamber assay. The Boyden Chamber system uses a two-chamber plate model in which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are stained and quantified. Most cells are sized from 30 to 50 µm can migrate through 3 to 10 µm pore.

产品信息
部件	3. Calcein-AM: (Part No. CS202541) One 50 µg vial. ECM221-1 1. 24-well Cell Migration Plate Assembly - 5 µm: (Part No. 2005708) Two 24-well plates, each containing 12- 5 µm pore inserts per plate. 2. BSA 30% solution: (Part No. CS203352) One vial containing 0.5 mL of a 30% solution. 3. 24-well Stain Receiver Plate: (Part No. PIMWS2450) One each. 4. 96-Well Black Quantitation Plate: (Part No. CR201763) One each. 5. Forceps: 1 pair ECM221-2 1. Laminin: (Part No. CS202685) Two vials each containing 60 μg in solution. 2. Accutase™: (Cat. No. SCR005) One 100 mL bottle of cell detachment solution.
检测方法	Fluorescent

产品信息

部件

3. Calcein-AM: (Part No. CS202541) One 50 µg vial.
ECM221-1
1. 24-well Cell Migration Plate Assembly - 5 µm: (Part No. 2005708) Two 24-well plates, each containing 12- 5 µm pore inserts per plate.
2. BSA 30% solution: (Part No. CS203352) One vial containing 0.5 mL of a 30% solution.
3. 24-well Stain Receiver Plate: (Part No. PIMWS2450) One each.
4. 96-Well Black Quantitation Plate: (Part No. CR201763) One each.
5. Forceps: 1 pair
ECM221-2
1. Laminin: (Part No. CS202685) Two vials each containing 60 μg in solution.
2. Accutase™: (Cat. No. SCR005) One 100 mL bottle of cell detachment solution.

检测方法

Fluorescent

应用
应用	This QCM Laminin Migration Assay incorporates a 5 µm porous membrane Boyden chamber & mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin.
应用说明	The Millipore QCM Laminin Migration Assay incorporates a 5 µm porous membrane Boyden chamber and mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin. The laminin is isolated from mouse sarcoma and it is enriched with mouse laminin 1 (laminin-111). This ECM promotes cell adhesion, proliferation as well as migration in many cell types such as embryonic stem cells, neural cells, liver cells and cancer cells. Cells interact with laminin through integrins α1β1, α2β2, α3β1, α6β1, α7β1, and α6β4. The assay is designed specifically to examine the migration of a subset of fibroblasts, cancer cells and neural cells. Laminin protein is coated on the bottom of each chamber’s porous membrane as the driver for cell migration. A similarly BSA-coated control chamber(s) provides an appropriate migration control. Cells that migrate toward laminin are stained using Calcein-AM™ a living cell fluorescent dye, quantified on a fluorescent microplate reader. Each kit provides sufficient materials for the evaluation of 24 samples.

应用

This QCM Laminin Migration Assay incorporates a 5 µm porous membrane Boyden chamber & mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin.

应用说明

The Millipore QCM Laminin Migration Assay incorporates a 5 µm porous membrane Boyden chamber and mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin. The laminin is isolated from mouse sarcoma and it is enriched with mouse laminin 1 (laminin-111). This ECM promotes cell adhesion, proliferation as well as migration in many cell types such as embryonic stem cells, neural cells, liver cells and cancer cells. Cells interact with laminin through integrins α1β1, α2β2, α3β1, α6β1, α7β1, and α6β4. The assay is designed specifically to examine the migration of a subset of fibroblasts, cancer cells and neural cells.
Laminin protein is coated on the bottom of each chamber’s porous membrane as the driver for cell migration. A similarly BSA-coated control chamber(s) provides an appropriate migration control. Cells that migrate toward laminin are stained using Calcein-AM™ a living cell fluorescent dye, quantified on a fluorescent microplate reader.

Each kit provides sufficient materials for the evaluation of 24 samples.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	ECM221 should be used within four months from date of receipt. In that time: • ECM221-1 kit components should be stored at 2° to 8°C. DO NOT FREEZE. • ECM221-2 kit components must be stored at -20°C until needed.