merck millipore,默克密理博,06-747,Anti-Neomycin Phosphotransferase II Antibody

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merck millipore,默克密理博,06-747,Anti-Neomycin Phosphotransferase II Antibody
产品名称：Anti-Neomycin Phosphotransferase II Antibody
产品型号：06-747
Anti-Neomycin Phosphotransferase II Antibody is an antibody against Neomycin Phosphotransferase II for use in WB.

merck millipore,默克密理博,06-747,Anti-Neomycin Phosphotransferase II Antibody

产品介绍

merck millipore,默克密理博,06-747,Anti-Neomycin Phosphotransferase II Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Bact	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-747
品牌系列	Upstate
商名	Upstate
描述	Anti-Neomycin Phosphotransferase II Antibody
背景信息	Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

产品信息
格式	Purified
控制	RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene
演示	Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

应用
应用	Anti-Neomycin Phosphotransferase II Antibody is an antibody against Neomycin Phosphotransferase II for use in WB.
主要应用	Western Blotting
应用说明	Western Blot Analysis: 1-2 μg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.

生物信息
免疫原品种	Full length neomycin phosphotransferase (NPT) II
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes NPT II, Mr ~30 kDa
同种型	IgG
品种反应性	Bacteria
Species Reactivity Note	Human. Expected to react with mouse and rat based on sequence homology.
抗体类型	Polyclonal Antibody
修改	Phosphorylation
纯化方法	Protein A purfied
UniProt编号	P00552

产品使用声明
质量保证	Routinely evaluated by Western Blot on transiently transfected Cos-1 cells. Western Blot Analysis: 1-2 μg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.