• merck millipore,默克密理博,SCR004,Alkaline Phosphatase Detection Kit
  • merck millipore,默克密理博,SCR004,Alkaline Phosphatase Detection Kit

    产品名称:Alkaline Phosphatase Detection Kit
    产品型号:SCR004
    This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.

    merck millipore,默克密理博,SCR004,Alkaline Phosphatase Detection Kit

  • 产品介绍
  • merck millipore,默克密理博,SCR004,Alkaline Phosphatase Detection Kit

    重要规格表

    主要应用
    Cult
    描述
    产品目录编号 SCR004
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Alkaline Phosphatase Detection Kit
    概述 Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
    产品信息
    部件
    • Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
    • Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
    应用
    应用 This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
    主要应用
    • Stem Cell Culture
    应用说明 Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

    Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

    Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

    For Research Use Only; Not for use in diagnostic procedures
    生物信息
    品种反应性 Mammals
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

    When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
    包装信息
    数量 100 assays

    merck millipore,默克密理博,SCR004,Alkaline Phosphatase Detection Kit

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