- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Sh ELISA Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1840 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Interleukin-8 Antibody Alternate Names - IL-8
产品信息 格式 Serum 演示 Rabbit serum. Liquid containing 0.1% sodium azide. 应用 应用 Anti-Interleukin-8 Antibody detects level of Interleukin-8 & has been published & validated for use in ELISA. 主要应用 - ELISA
应用说明 Detecting antibody in ELISA, using monoclonal anti-ovine IL-8 as capture antibody. Recommended dilution factor 1:500. See suggested protocol.
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-8
1. Coat high capacity microtiter wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.生物信息 免疫原品种 Recombinant ovine IL-8 宿主 Rabbit 特异性 Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP or TNFalpha. 品种反应性 Sheep 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000584.2
Entrez基因汇总 The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. 基因符号 - NAF
- IL8
- GCP-1
- emoctakin
- MDNCF
- NAP1
- CXCL8
- AMCF-I
- IL-8
- Emoctakin
- TSG-1
- NAP-1
- LYNAP
- MONAP
- SCYB8
- GCP1
- LUCT
- LECT
- K60
- 3-10C
- b-ENAP
UniProt编号 - P10145
UniProt汇总 FUNCTION: SwissProt: P10145 # IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
SIZE: 99 amino acids; 11098 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
PTM: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
SIMILARITY: SwissProt: P10145 ## Belongs to the intercrine alpha (chemokine CxC) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,AB1840,Anti-Interleukin-8 Antibody
下一件merck millipore产品：merck millipore,默克密理博,203297,Bisindolylmaleimide IV - CAS 119139-23-0 - Calbiochem
相关产品
merck millipore,默克密理博,71074,BetaBlue™ Staining Kit
merck millipore,默克密理博,07-1584,Anti-dimethyl Histone H4 (Lys20) Antibody
merck millipore,默克密理博,553502,Rac1 Inhibitor - CAS 1177865-17-6 - Calbiochem
merck millipore,默克密理博,MABT152,Anti-Kinesin-like protein KIF11 Antibody, clone 14B12.2
merck millipore,默克密理博,AB1840,Anti-Interleukin-8 Antibody
merck millipore,默克密理博,203297,Bisindolylmaleimide IV - CAS 119139-23-0 - Calbiochem
merck millipore,默克密理博,14-811,UbcH5c Conjugating Enzyme, 100 µg
merck millipore,默克密理博,AP132-KC,Goat Anti-Rabbit IgG, H + L Affinity Purified (10mg) KC
merck millipore,默克密理博,681668,Wnt Synergist, QS11 - CAS 944328-88-5 - Calbiochem
merck millipore,默克密理博,AB5908-200UL,Anti-Potassium Channel HERG Antibody, CT