merck millipore,默克密理博,14-811,UbcH5c Conjugating Enzyme, 100 µg

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Merck Millipore 默克密理愽

Entrez基因编号	品种	Uni Prot编号
NM_181893.1, NM_003340.4, NM_181886.1, NM_181887.1, NM_181888.1, NM_181889.1, NM_181890.1, NM_181891.1, NM_181892.1	Human	P61077

描述
产品目录编号	14-811
品牌系列	Upstate
商名	Upstate
描述	UbcH5c Conjugating Enzyme, 100 µg
概述	The His6-tagged fusion protein of UbcH5c is charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation. UbcH5c is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53, NF-κB, and c-Fos. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.

应用
应用	The His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.

生物信息
纯度	>95% by SDS-PAGE
来源	Recombinant human UbcH5c Conjugating Enzyme expressed in E. coli.
品种	Human
Entrez基因编号	NM_181893.1 NM_003340.4 NM_181886.1 NM_181887.1 NM_181888.1 NM_181889.1 NM_181890.1 NM_181891.1 NM_181892.1
Entrez基因汇总	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
基因符号	MGC5416 UBE2D3 UBCH5C MGC43926 E2 UBC4/5 UbcH5C E2(17)KB3
UniProt编号	P61077
UniProt汇总	FUNCTION: SwissProt: P61077 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. SIZE: 147 amino acids; 16687 Da SIMILARITY: SwissProt: P61077 ## Belongs to the ubiquitin-conjugating enzyme family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.

包装信息
数量	100 µg

merck millipore,默克密理博,14-811,UbcH5c Conjugating Enzyme, 100 µg

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