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merck millipore,默克密理博,07-1584,Anti-dimethyl Histone H4 (Lys20) Antibody
产品名称：Anti-dimethyl Histone H4 (Lys20) Antibody
产品型号：07-1584
Anti-dimethyl Histone H4 (Lys20) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl Histone H4 (Lys20) also known as H4K20me2, Histone H4 (di methyl K20) & has been validated in WB.
merck millipore,默克密理博,07-1584,Anti-dimethyl Histone H4 (Lys20) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1584 品牌系列 Upstate
商名 - Upstate
描述 Anti-dimethyl Histone H4 (Lys20) Antibody Alternate Names - Histone H4 (di methyl K20)
- H4K20me2
背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). In mammals, H4K20 methylation is one of the most common chromatin modifications and it has been implicated in various biological processes, including heterochromatin formation, telomere function, imprinting, X inactivation, DNA damage repair and cancer progression. H4K20me2 is the most abundant histone modification with more that 80% of all histone H4 molecules carrying this mark. The Suv4-20h enzymes are the two major histone methyltransferases to induce H4K20me2 and me3 states. 产品信息 格式 Purified 应用 应用 Anti-dimethyl Histone H4 (Lys20) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl Histone H4 (Lys20) also known as H4K20me2, Histone H4 (di methyl K20) & has been validated in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptide containing the sequence (AKRHR[dimethyl-K]VLRD-C), corresponding to the sequence surrounding acetylated lysine 20 of human histone H4. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes histone H4 acetylated on Lys20, Mr ~10 kDa. 品种反应性 Human Species Reactivity Note Broad species cross-reactivity expected due to sequence homology 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_175054
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. 修改 - Methylation
UniProt编号 - P62805
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
INTERACTION:
Q13547:HDAC1; NbExp=1; IntAct=EBI-302023, EBI-301834;
P56524:HDAC4; NbExp=1; IntAct=EBI-302023, EBI-308629;
Q9UQL6:HDAC5; NbExp=1; IntAct=EBI-302023, EBI-715576;
Q9UBN7:HDAC6; NbExp=1; IntAct=EBI-302023, EBI-301697;
Q9Y468:L3MBTL; NbExp=2; IntAct=EBI-302023, EBI-1265089;
Q9SU94:PRMT1.1 (xeno); NbExp=1; IntAct=EBI-302023, EBI-1382731;
O82210:PRMT1.2 (xeno); NbExp=1; IntAct=EBI-302023, EBI-1382526;
Q9NQR1:SETD8; NbExp=4; IntAct=EBI-302023, EBI-1268946;
SUBCELLULAR LOCATION: Nucleus.
PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.
PTM: Citrullination at Arg-4 by PADI4 impairs methylation.
PTM: Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.
PTM: Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
PTM: Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
PTM: Sumoylated, which is associated with transcriptional repression.
SIMILARITY: Belongs to the histone H4 family.
产品使用声明 质量保证 Routinely evaluated by immunoblot on HeLa nuclear extract 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
包装信息 数量 100 µg
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