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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1730 Replaces 04-438 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Bid (full length) Antibody Alternate Names - BH3 Interacting Domain Death Agonist
背景信息 Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin in liquid in PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. 应用 应用 This Anti-Bid (full length) Antibody is validated for use in IP, WB, IH for the detection of Bid (full length). 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blot: 1 μg/mL
Immunoprecipitation: 5-10 μg/mL
Immunohistochemistry: 20-40 μg/mL
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 An 18 amino acid synthetic peptide peptide surrounding amino acid 37 of human Bid (ID# BV63-64), corresponding to a region near the N-terminus of human Bid. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB1730 detects the full length (22 kDa), detection of the 15kDa truncated form is very difficult with AB1730. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_197967.1
- NM_001196.2
- NM_197966.1
Entrez基因汇总 This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. 基因符号 - MGC15319
- BID
- FP497
- MGC42355
UniProt编号 - P55957
UniProt汇总 FUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SIZE: 195 amino acids; 21995 Da
SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic. & BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitochondria as an integral membrane protein (By similarity). & BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note=Associated with the mitochondrial membrane (By similarity). & Isoform 1: Cytoplasm. & Isoform 3: Cytoplasm. & Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
TISSUE SPECIFICITY: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
DOMAIN: SwissProt: P55957 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY:产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C for up to 12 momnths from date of receipt. For optimal storage, aliquot and freeze at -20°C to -70°C. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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