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merck millipore,默克密理博,DR1002,PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)
产品名称：PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)
产品型号：DR1002
This PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).
merck millipore,默克密理博,DR1002,PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992. 产品目录编号 DR1002 品牌系列 Calbiochem®
同义词 Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹) 应用数据 
Detection of human ATM phosphorylated at Ser1981 by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.
Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.参考 参考 Horejsi, Z., et al. 2004. Oncogene 23, 3122.
Abraham, R.T. 2003. Genes Dev. 15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.产品信息 声明 Sold under license of U.S. Patent 7,108,992. 形式 Liquid 配方 In PBS. 正控制 HeLa cells treated with camptothecin (Cat. No. 208925) 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
生物信息 免疫原 a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM 免疫原品种 Human 克隆 10H11.E12 宿主 Mouse 同种型 IgG₁ 品种 human, mouse 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Blue Ice Only
毒性 Irritant
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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