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品种反应性 主要应用 宿主 格式 抗体类型 M WB R Purified Monoclonal Antibody 描述 产品目录编号 MABC604 描述 Anti-MLKL Antibody, clone 3H1 Alternate Names - MLKL
- Mixed lineage kinase domain-like protein
背景信息 MLKL, also known as Mixed lineage kinase domain-like protein, and encoded by the gene MLKL, is an interesting protein that is required for the execution of programmed necrosis or necroptosis, for example in response to TNF-alpha induced necrosis. MLKL is a component of the "necrosome," the multiprotein complex that triggers tumor necrosis factor (TNF)-induced cell death by necroptosis. The programmed necrosis induced by TNF-α also requires the activities of the receptor-interacting serine-threonine kinases RIP1 and RIP3 and they too interact directly with the mixed lineage kinase domain-like protein MLKL. RIP3 is an essential upstream kinase in necroptosis. The pseudokinase MLKL functions as a substrate of RIP3 to mediate downstream signaling and when complexed to MLKL the RIP3-MLKL complex is stabilized by AMP-PNP to adopt an inactive conformation. 产品信息 格式 Purified 演示 Purified rat monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-MLKL Antibody, clone 3H1 is validated for use in western blotting, IP & immunofluorescence for the detection of MLKL. 主要应用 - Western Blotting
应用说明 Western Blotting Analysis: A representative lot from an independent laboratory detected MLKL in wild type mouse tissues and not in MLKL knock out mouse tissues (Murphy, J. M., et al. (2013). Immunity. 39(3).443-453.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated MLKL in transfected MDF cell lysates (Murphy, J. M., et al. (2013). Immunity. 39(3).443-453.).
Immunofluorescence Analysis: A representative lot from an independent laboratory in mouse dermal fibroblasts (Prof. James M. Murphy, Walter and Eliza Hall Institute.).生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to mouse MLKL. 克隆 3H1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 同种型 IgG 品种反应性 Mouse Species Reactivity Note Reacts with mouse. Expected to react with human, chimpanzee, rat, bovine, equine and Rhesus Monkey due to sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_083281
基因符号 - Mlkl
纯化方法 Protein G purified UniProt编号 - Q9D2Y4
产品使用声明 质量保证 Evaluated by Western Blotting in mouse heart tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MLKL in 10 µg of mouse heart tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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