• merck millipore,默克密理博,69067,Enterokinase Cleavage Capture Kit
  • merck millipore,默克密理博,69067,Enterokinase Cleavage Capture Kit

    产品名称:Enterokinase Cleavage Capture Kit
    产品型号:69067


    merck millipore,默克密理博,69067,Enterokinase Cleavage Capture Kit

  • 产品介绍
  • merck millipore,默克密理博,69067,Enterokinase Cleavage Capture Kit

    描述
    概述 The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase.
    Following cleavage of the target protein, rEK is removed with > 99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration. Because the same buffer conditions are used for both cleavage and capture, no buffer changes are necessary.

    The kit also includes a Cleavage Control Protein for conducting control digests in parallel with experimental samples, or to test cleavage under customized buffer conditions. The 48 kDa Cleavage Control Protein is cleaved into two proteolytic fragments of 32 kDa and 16 kDa, which are easily visualized by standard SDS-PAGE followed by Coomassie blue staining (see figure below). The Cleavage Control Protein also features an amino terminal S•Tag™ sequence enabling sensitive detection of the 16 kDa proteolytic product with Western Blot reagents. The Cleavage Control Protein is also available separately.

    产品目录编号 69067
    品牌系列 Novagen®
    参考
    参考 1. Collins-Racie, L.A., McColgan, J.M., Grant, K.L., DiBlasio-Smith, E.A., McCoy, J.M., and LaVallie, E.R. (1995) Bio/Technology 13, 982–987.
    产品信息
    部件
    储存和货运信息
    货运代码 Blue Ice Only
    毒性 Multiple Toxicity Values, refer to MSDS
    储存 Multiple storage requirements
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No

    merck millipore,默克密理博,69067,Enterokinase Cleavage Capture Kit

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