merck millipore,默克密理博,07-327,Anti-acetyl-Histone H4 (Lys5) Antibody

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merck millipore,默克密理博,07-327,Anti-acetyl-Histone H4 (Lys5) Antibody
产品名称：Anti-acetyl-Histone H4 (Lys5) Antibody
产品型号：07-327
Use Anti-acetyl-Histone H4 (Lys5) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, ICC, ChIP-seq, ChIP to detect acetyl-Histone H4 (Lys5) also known as H4K5Ac, Histone H4 (acetyl K5).

merck millipore,默克密理博,07-327,Anti-acetyl-Histone H4 (Lys5) Antibody

产品介绍

merck millipore,默克密理博,07-327,Anti-acetyl-Histone H4 (Lys5) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ChIP, WB, ICC	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	07-327
描述	Anti-acetyl-Histone H4 (Lys5) Antibody
Alternate Names	Histone H4
背景信息	Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

产品信息
格式	Serum
控制	HeLa acid extract
演示	Immunodepleted rabbit serum with 0.05% soduim azide and 30% glycerol.

应用
应用	Use Anti-acetyl-Histone H4 (Lys5) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, ICC, ChIP-seq, ChIP to detect acetyl-Histone H4 (Lys5) also known as H4K5Ac, Histone H4 (acetyl K5).
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl-Histone H4 in HeLa and A431 cells. Dot Blot Specificity Analysis: A representative lot detected acetyl-histone H4 (Lys5) peptides, but not unacetylated-histone H4 (Lys4) peptides or other peptides corresponding to modified histones. Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated acetyl-Histone H4 in HeLa S3 chromatin.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to the N-terminus of Histone H4 acetylated at Lys5.
表位	N-terminus
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes the N-terminus of Histone H4 acetylated at Lys5.
品种反应性	Human
Species Reactivity Note	Demonstrated to react with Human based on 100% sequence homology. This antibody is expected to demonstrate broad species cross-reactivity.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001029249
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
基因符号	HIST4H4 HIST1H4A H4/A H4FA HIST1H4B H4/I H4FI HIST1H4C H4/G H4FG HIST1H4D H4/B H4FB HIST1H4E H4/J H4FJ HIST1H4F H4/C H4FC HIST1H4H H4/H H4FH HIST1H4I H4/M H4FM HIST1H4J H4/E H4FE HIST1H4K H4/D H4FD HIST1H4L H4/K H4FK HIST2H4A H4/N H4F2 H4FN HIST2H4 HIST2H4B H4/O H4FO
修改	Acetylation
纯化方法	Purified by immunodepletion
UniProt编号	P62805
UniProt汇总	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. Chromosome.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H4 in 10 µg of HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.