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品种反应性 主要应用 宿主 格式 抗体类型 R, M IHC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1722 Replaces 04-436 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 背景信息 In C. elegans, ced-3 and ced-4 were identified as genes essential for programmed cell death, while ced-9 was found to be a regulator of cell death by preventing apoptosis. The first mammalian homolog for ced-3 was described in 1988 as Bcl-2 which is involved in B-cell lymphomas (Vaux and Adams, 1988, Nature 335: 440). Bcl-2 transfected B cells were shown to be resistant towards apoptosis normally induced in B cells by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis. The bcl-2 gene codes for a 25 kDa protein. The C terminal 21 amino acids encode a stretch of hydrophobic amino acids that are important in membrane docking: Bcl-2 resides on the cytoplasmic face of the mitochondrial outer membrane, the nuclear envelop, and the endoplasmic reticulum. Deletion of the C terminus does not abrogate Bcl-2 survival function! Most Bcl-2 homologs have this hydrophobic C terminal domain, though they not necessarily are located on membranes but are cytosolic (e.g. Bax). 产品信息 格式 Serum 演示 Whole serum without preservatives 应用 应用 Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 is an antibody against Bcl-2 for use in WB, IP, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunoblotting: Recommended antibody dilution 1:1500-3000
Immunoprecipitation: Recommended antibody dilution 1:50
Immunohistochemistry: Recommended antibody dilution 1:600-1500
Optimal antibody dilutions must be determined by end-user.生物信息 免疫原品种 Peptide against mouse Bcl-2 a.a. 68-86 RTSPLRPLVATAGPALSPV 表位 a.a. 68-86 of mBCL2 宿主 Rabbit 特异性 AB1722 recognizes Bcl-2 alpha and beta isoforms in mouse and rat. Cross reactivity with other species is unknown 品种反应性 RatMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000657.2
- NM_000633.2
Entrez基因汇总 This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. 基因符号 - Bcl-2
- BCL2
UniProt编号 - P10415
UniProt汇总 FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
SIMILARITY: Belongs to the Bcl-2 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze thaws. 包装信息 数量 100 µL
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