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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB M Purified Monoclonal Antibody 描述 产品目录编号 05-535 品牌系列 Upstate
商名 - Upstate
描述 Anti-IKKβ Antibody, clone 10AG2 Alternate Names - inhibitor of nuclear factor kappa B kinase beta subunit
- I-kappa-B kinase 2
- I-kappa-B-kinase beta
- Nuclear factor NF-kappa-B inhibitor kinase beta
- inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
- kinase beta
背景信息 IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1. 产品信息 格式 Purified 控制 - Jurkat cell lysate and human cervical carcinoma.
Included Positive Antigen Control: Catalog # 12-303, Jurkat cell extract. Use 20 µg per lane for minigels.
演示 Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.05% BSA, with 0.05% sodium azide. Frozen solution. 应用 应用 Anti-IKKβ Antibody, clone 10AG2 detects level of IKKβ & has been published & validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 His-tagged, full length human IKKβ/IKK2. Clone: 10AG2. 克隆 10AG2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes IKKβ/IKK2, Mr 87 kDa. 同种型 IgG1 品种反应性 MouseHumanRat Species Reactivity Note Proven to react with human, mouse and rat. Other species not tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001556
Entrez基因汇总 NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] 基因符号 - IkBKB
- IKBKB
- IKK-beta
- NFKBIKB
- IKK2
- FLJ40509
- IKKB
- MGC131801
- IKK-B
纯化方法 Protein G purfied UniProt编号 - O14920
UniProt汇总 FUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity).
SIZE: 756 amino acids; 86564 Da
SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by western blot on human Jurkat RIPA cell lysate, human A431, HeLa nuclear extract, mouse 3T3/A31 or rat PC-12 RIPA cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected IKK/IKK2 in human HeLa cell lysate. A previous lot was tested in human Jurkat RIPA cell lysate, A431, extract, mouse 3T3/A31 and rat PC-12 RIPA cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 100 µg
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