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纯度 ≥95% by SDS-PAGE 描述 概述 Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml. 产品目录编号 371207 品牌系列 Calbiochem®
参考 参考 Pinzone, J.J., et al. 2009. Blood 113, 517.
Li, J., et al. 2006. Bone 36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C., 2006. Oncogene 25, 7469.
Verani, R., et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M., et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B., et al. 2002. Nature 417, 664.
Mao, B., et al. 2001. Nature 411, 321.
Glinka, A., et al. 1998. Nature 391, 357.产品信息 形式 Liquid 配方 Lyophilized from 0.2 µm sterile-filtered PBS, BSA. 生物信息 生物活性 ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. 纯度 ≥95% by SDS-PAGE 理化信息 污染物 Endotoxin: <1.0 EU/µg protein 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
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