merck millipore,默克密理愽
  • merck millipore,默克密理博,NS220,Neurite Outgrowth Assay Kit (3 µm)

  • merck millipore,默克密理博,NS220,Neurite Outgrowth Assay Kit (3 µm)

    产品名称：Neurite Outgrowth Assay Kit (3 µm)
    产品型号：NS220
    The NS220 Neurite Outgrowth Assay Kit (3 µm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base.

    merck millipore,默克密理博,NS220,Neurite Outgrowth Assay Kit (3 µm)

  • 产品介绍
  • merck millipore,默克密理博,NS220,Neurite Outgrowth Assay Kit (3 µm)

    重要规格表

    主要应用 检测方法
    NeuroscienceChromogenic
    描述
    产品目录编号 NS220
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Neurite Outgrowth Assay Kit (3 µm)
    概述 Application

    CHEMICON's NS220 Neurite Outgrowth Assay Kit (3 µm) utilizes microporous tissue culture insert technology from Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm diameter, e.g. N1E-115 cells [Abe et al., 2003], Dorsal Root Ganglia and Schwann cells [Windebank et al., 1985]. The inserts are designed to fit into a receiver vessel, which when in use contains differentiation media in contact with the bottom of the insert membrane. The permeable membranes allow for discrimination between neurites and cell bodies, as projecting neurites will pass easily through the pores but cell bodies will not. Therefore, by inducing neurites to traverse these membrane pores, a purified population of neurites is located on the underside of the membrane, distal to the surface on which neural cell bodies are deposited.
    The assay is a simple, efficient, and versatile system for the quantitative determination of compounds that influence neurite formation and repulsion. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsible for neurite extension and repulsion, as well as analyze gene function in transfected cells. The microporous filter allows for biochemical separation and purification of neurites and cell bodies for detailed molecular analysis of protein expression, signal transduction processes and identification of drug targets that regulate neurite outgrowth or retraction processes. Reagents and materials supplied in the NS220 Neurite Outgrowth Assay Kit are sufficient for 12 tests.
    Note: the 3 µm pore size inserts in this kit are unsuitable for use with PC12 cells. Due to the relatively small diameter of PC12 cell bodies [Abe et al., 2003], some of these cells may pass though 3 µm pores and obscure the results. Chemicon's NS225 Neurite Outgrowth Assay Kit (1 µm) has been specifically designed for and validated with PC12 cells.
    产品信息
    部件
    • 6. Forceps: (Part No. 10203) One each.
    • 1. Neurite Outgrowth Plate Assembly, 3 μm: (Part No. 2007256) 1 x 24-well plate containing 12 x Millicell hanging inserts with 3 μm pore size membranes.
    • 2. Neurite Stain Solution: (Part No. 90242) One 20 mL bottle.
    • 3. Neurite Stain Extraction Buffer: (Part No. 90243) One 20 mL bottle.
    • 4. Neurite Outgrowth Assay Plate: (Part No. 2007255) Two 24-well plates.
    • 5. Cotton Swabs: (Part No. 10202) 50 swabs.
    检测方法 Chromogenic
    应用
    应用 The NS220 Neurite Outgrowth Assay Kit (3 µm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base.
    主要应用
    • Neuroscience
    生物信息
    品种反应性 All
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 When stored at 2-8° C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
    包装信息
    数量 12 assays

    merck millipore,默克密理博,NS220,Neurite Outgrowth Assay Kit (3 µm)

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