merck millipore,默克密理博,341292,PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

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Merck Millipore 默克密理愽

宿主
Rb

描述
概述	Recognizes the ~125 kDa FAK protein when phosphorylated at Tyr³⁹⁷. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
产品目录编号	341292
品牌系列	Calbiochem®
同义词	Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
应用数据	Detection of FAK, phosphorylated on Tyr³⁹⁷, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.

参考
参考	Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235. Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758. Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.

产品信息
形式	Liquid
配方	In Dulbecco's PBS (no Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.
正控制	Chicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
防腐剂	≤0.1% sodium azide

应用
主要应用	Immunoblotting (Western Blotting) Immunohistochemistry

生物信息
免疫原	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
宿主	Rabbit
同种型	IgG
品种	Drosophila, chicken, frog, human, mouse, rat

储存和货运信息
货运代码	Dry Ice Only
毒性	Standard Handling
储存	≤ -70°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following initial thaw, aliquot and freeze (-20°C).

merck millipore,默克密理博,341292,PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

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