merck millipore,默克密理博,04-1149,Anti-ILK Antibody, clone EPR1592, rabbit monoclonal

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merck millipore,默克密理博,04-1149,Anti-ILK Antibody, clone EPR1592, rabbit monoclonal
产品名称：Anti-ILK Antibody, clone EPR1592, rabbit monoclonal
产品型号：04-1149
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ILK Antibody, clone EPR1592, Rabbit is val More>>

merck millipore,默克密理博,04-1149,Anti-ILK Antibody, clone EPR1592, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1149,Anti-ILK Antibody, clone EPR1592, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB, ICC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-1149
描述	Anti-ILK Antibody, clone EPR1592, rabbit monoclonal
Alternate Names	integrin-linked kinase 59 kDa serine/threonine-protein kinase
背景信息	ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).

产品信息
格式	Culture Supernatant
控制	HeLa, 293T and Jurkat cell lysates
演示	Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ILK Antibody, clone EPR1592, Rabbit is validated for use in WB, IC, FC for the detection of ILK.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot detected ILK in HeLa cell lysate. Flow Cytometry: 1:60 dilution from a previous lot of this antibody was used in FC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in human ILK.
克隆	EPR1592
宿主	Rabbit
特异性	This antibody recognizes ILK.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	Q13418
基因符号	p59ILK DKFZp686F1765 EC 2.7.11.1 ILK-1 ILK-2 ILK1 ILK2 P59
纯化方法	Unpurified
UniProt编号	Q13418
UniProt汇总	FUNCTION: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK. SUBUNIT STRUCTURE: Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1. SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. TISSUE SPECIFICITY: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. DOMAIN: A PH-like domain is involved in phosphatidylinositol phosphate binding. PTM: Autophosphorylated on serine residues. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 5 ANK repeats. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa, 293T and Jurkat cell lysates. Western Blot Analysis: 1:5,000 - 50,000 dilution of this antibody was used to detect ILK in HeLa, 293T and Jurkat cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.