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宿主 M 描述 概述 Recognizes the ~62 kDa Plk1 protein in HeLa cells. 产品目录编号 DR1037 品牌系列 Calbiochem®
同义词 Anti-Polo-Like Kinase 应用数据 
Detection of human Plk1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells treated with nocodazole (100 ng/ml). Primary antibody: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (1 µg/ml). Detection: chemiluminescence.
Detection of human Plk1 by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (446 µg) from HeLa cells treated with nocodazole (100 ng/ml). Antibody for IP: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (5 µg). Conditions for immunoblotting: same as above.参考 参考 Takai, N., et al. 2005. Oncogene 24, 287.
Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
Glover, D.M., et al. 1998. Genes Devel. 12, 3777.产品信息 形式 Liquid 配方 In PBS. 正控制 Nocodazole-treated HeLa cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 full-length, recombinant, human Plk1 免疫原品种 Human 克隆 35-206 宿主 Mouse 同种型 IgG2b 品种 human, mouse, rat 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Blue Ice Only
毒性 Irritant
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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