merck millipore,默克密理博,MABE351,Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ChIP, WB, ICC	M	Ascites	Monoclonal Antibody

描述
产品目录编号	MABE351
描述	Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5
Alternate Names	H3/g Histone H3.1t H3/t H3t
背景信息	Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, acetylation of H3K4 is regulated by Gcn5 and the Mst2 complex, and is crucial to the activation of DNA damage checkpoint.

产品信息
格式	Ascites
控制	HeLa acid extract
演示	Mouse monoclonal IgG1 in ascites containing 0.05% sodium azide.

应用
应用	Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation Analysis: A representative lot was used to immunoprecipitate acetyl-Histone H3 (Lys4) in IP. Immunocytochemistry Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ICC. Chromatin Immunoprecipitation Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ChIP. Western Blot Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in WB.

生物信息
免疫原品种	Ovalbumin-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys14.
克隆	clone 13HH3-1A5
宿主	Mouse
同种型	IgG1
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with Human. Demonstrated to react in Mouse ESCs in ChIP-seq in Karmodiya et al. BMC Genomics 2012, 13:424.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003484
基因符号	H3FT HIST3H3
纯化方法	Unpurified
UniProt编号	Q16695

产品使用声明
质量保证	Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H3 (Lys4) in 10 µg of HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

包装信息
数量	100 µL

merck millipore,默克密理博,MABE351,Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

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