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merck millipore,默克密理博,324864,PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)
产品名称：PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)
产品型号：324864
This PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of EGFR (pTyr¹¹⁷³).
merck millipore,默克密理博,324864,PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes EGFR phosphorylated at Tyr1173 in vanadate-treated HepG2 cells. A positive control is provided. 产品目录编号 324864 品牌系列 Calbiochem®
同义词 Anti-Epidermal Growth Factor Receptor (pTyr¹¹⁷³) 应用数据 
Detection of human EGFR, phosphorylated on Tyr1173, by immunoblotting. Samples: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) A549 cells. Primary antibody: PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) (Cat. No. 324864) (0.5 µg/ml). Detection: chemiluminescence.产品信息 形式 Lyophilized 配方 100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate. 正控制 Vanadate-treated HepG2 cells (included) 防腐剂 ≤0.1% sodium azide (antibody only) 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 a synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR 免疫原品种 Human 克隆 9H2 宿主 Mouse 同种型 IgG₁ 品种 human, mouse, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Reconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
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