- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,07-528,Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 Antibody
产品名称：Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 Antibody
产品型号：07-528
Use Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 Antibody (rabbit polyclonal antibody) validated in WB, PIA, Mplex, DB to detect trimethyl (Lys79)-phospho (Thr80)-Histone H3 also known as H3K79me More>>
merck millipore,默克密理博,07-528,Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-528 品牌系列 Upstate
商名 - Upstate
描述 Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 Antibody Alternate Names - Histone H3 (di methyl K79, phospho T80)
- H3K79me2T80P
- Histone H3 (di methyl K79
产品信息 格式 Affinity Purified 演示 0.1 M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30% 应用 应用 Use Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 Antibody (rabbit polyclonal antibody) validated in WB, PIA, Mplex, DB to detect trimethyl (Lys79)-phospho (Thr80)-Histone H3 also known as H3K79me2T80P, Histone H3 (di methyl K79 phospho T80). 主要应用 - Western Blotting
生物信息 免疫原品种 peptide containing the sequence [trimKpT] in which lysine 79 is trimethylated and threonine 80 is phosphorylated on human histone H3 and histone H4. 宿主 Rabbit 特异性 histone H3 trimethylated at lysine 79 and phosphorylated at threonine 80 同种型 IgG 品种反应性 Human Species Reactivity Note Broad species reactivity is expected. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003493
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. 基因符号 - H3F3B
- H3F3A
- MGC87783
- H3.3A
- MGC87782
- H3F3
- H3.3B
修改 - Phosphorylation
- Methylation
纯化方法 Immunoaffinity purified UniProt编号 - Q16695
UniProt汇总 FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.产品使用声明 质量保证 routinely evaluated by immunoblot in acid-extracted proteins from HeLa cells arrested with colcemid to a greater extent than in untreated HeLa cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,ABN310,Anti-FA2H Antibody
下一件merck millipore产品：merck millipore,默克密理博,05-1143,Anti-phospho-Paxillin (Tyr31) Antibody, clone M102
相关产品
merck millipore,默克密理博,MAB10424,Anti-Plexin B3 Antibody, clone 17H10.1
merck millipore,默克密理博,ABT296,Anti-TOMM40 Antibody
merck millipore,默克密理博,324864,PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)
merck millipore,默克密理博,MAB5416,Anti-Glutamate Receptor 3 Antibody, clone 3B3
merck millipore,默克密理博,ABN310,Anti-FA2H Antibody
merck millipore,默克密理博,05-1143,Anti-phospho-Paxillin (Tyr31) Antibody, clone M102
merck millipore,默克密理博,HTS039LT,ChemiBrite™ D2L Dopamine Family Receptor Frozen Cells
merck millipore,默克密理博,324682,Elastase, Porcine Pancreas, High Purity, Crystallized
merck millipore,默克密理博,SF-1714,ICK; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,373275,Hh Signaling Antagonist XII, HPI-1 - CAS 599150-20-6 - Calbiochem