merck millipore,默克密理博,HTS039LT,ChemiBrite™ D2L Dopamine Family Receptor Frozen Cells

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,HTS039LT,ChemiBrite™ D2L Dopamine Family Receptor Frozen Cells
产品名称：ChemiBrite™ D2L Dopamine Family Receptor Frozen Cells
产品型号：HTS039LT
Human D2L GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS039LT,ChemiBrite™ D2L Dopamine Family Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS039LT,ChemiBrite™ D2L Dopamine Family Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	CHO (Chinese hamster ovary cells)	D2L

描述
产品目录编号	HTS039LT
商名	ChemiBrite
描述	ChemiBrite™ D_2L Dopamine Family Receptor Frozen Cells
背景信息	Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds. Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Dopamine is a catecholamine neurotransmitter that functions in the CNS to control locomotor, cognitive, emotional and neurendocrine processes, and in the periphery to modulate cardiovascular, renal and gastrointestinal processes. The biological activities of dopamine are mediated by a family of five GPCRs. The D1 and D5 subtypes couple to Gs to increase intracellular cAMP, whereas the D2, D3 and D4 subtypes couple to Gi to reduce cAMP (Missale et al., 1998). The D2 dopamine receptors have been of particular clinical interest due to their regulation of prolactin secretion and their affinity for antipsychotic drugs. The D2 receptor exists as two alternatively spliced isoforms differing in the insertion of a stretch of 29 amino acids in the third intracellular loop (D2S and D2L) (Giros et al., 1989; Grandy et al., 1989). Millipore’s cloned human D2L receptor-expressing ChemiBrite cells are made by transient transfection of CHO cells of optimized quantities of plasmids encoding the receptor and clytin and a promiscuous G protein to couple the receptor to the calcium signaling pathway and then cryopreserving the cells at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis of agonists, antagonists and modulators at the D2L receptor.

产品信息
部件	Vial contains 1 ml of mycoplasma-free cells

应用
应用	Human D2L GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_ 000795
GPCR类别	A
宿主细胞	CHO (Chinese hamster ovary cells)
蛋白靶	D2L
靶子系列	Dopamine

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.