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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1286 商名 - Upstate
描述 Anti-ATM Antibody Alternate Names - TEL1, telomere maintenance 1, homolog
- A-T, mutated
- AT mutated
- ataxia telangiectasia mutated
- ataxia telangiectasia mutated (includes complementation groups A, C and D)
- ataxia telangiectasia mutated protein
- ATM
- human phosphatidylinositol 3-kinase homolog
- serine-protein kinase ATM
- human phosphatidylinositol 3-kinase homolog
- serine-protein kinase ATM
- TEL1
- telomere maintenance 1
背景信息 ATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981. 产品信息 格式 Affinity Purified 控制 - HeLa nuclear extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ATM Antibody detects level of ATM & has been published & validated for use in WB & IF. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected ATM in 10 µg of HeLa nuclear extract.
Immunocytochemistry Analysis: A representative lot detected ATM in HeLa and NIH/3T3 cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected ATM in NIH/3T3 cells.生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to sequence derived from the Abl-interacting domain of human ATM. 表位 Abl-interacting domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Detects ATM. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human, Mouse. Predicted to react with Bovine, Rat, Horse based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000042.
Entrez基因汇总 The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1.
This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
基因符号 - TELO1
- AT1
- ATA
- ATC
- ATD
- ATDC
- ATE
- DKFZp781A0353 2
- EC 2.7.11.1 3
- MGC74674
- TEL1
纯化方法 Antigen affinity purified UniProt编号 - Q13315
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:500 dilution of this antibody detected ATM in 10 µg of HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.包装信息 数量 200 µL
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