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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN510 描述 Anti-AKAP Antibody1 Alternate Names - AKAP1
- A-kinase anchor protein 1, mitochondrial
- A-kinase anchor protein 149 kDa
- AKAP 149
- Dual specificity A-kinase-anchoring protein 1
- D-AKAP-1
- Protein kinase A-anchoring protein 1
- PRKA1
- Spermatid A-kinase anchor protein 84
- S-AKAP84
背景信息 The protein named A-kinase anchor protein 1, mitochondrial, or alternatively A-kinase anchor protein 149 kDa, (AKAP 149), Dual specificity A-kinase-anchoring protein 1 (D-AKAP-1), Protein kinase A-anchoring protein 1 (PRKA1), or Spermatid A-kinase anchor protein 84 (S-AKAP84) and encoded by the human gene named AKAP1/AKAP149/PRKA1 is a mitochondrial localized protein that binds protein kinase A (PKA) and anchors it to the cytoplasmic face of the mitochondrial outer membrane. AKAP1 is highly expressed in a variety of tissues and cancers. As an anchor for PKA, AKAP1 is involved in cAMP dependent signal transduction and may also play a role in RNA compartmentalization, something particularly important in neuronal and glial cell functions in the mature and developing brain. AKAP1 is also important in other cellular functions within the nervous system, including neuronal morphogenesis, dendrite and synapse development. EMD-Millipore’s Anti-AKAP1 rabbit polyclonal antibody has been tested in western blot on HeLa cell extracts, paraffin embedded immunohistochemistry on human prostate cancer sections, and fluorescent immunocytochemistry on MCF-7 cells in culture. 产品信息 格式 Affinity Purified 演示 Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol. 应用 应用 Detect AKAP using this rabbit polyclonal antibody, Anti-AKAP Antibody1 validated for use in western blotting, IHC & Immunofluorescence. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected AKAP1 in human prostate cancer tissue.
Immunofluorescence Analysis: A 1:25 dilution from a representative lot detected AKAP1 in MCF-7 cells.生物信息 免疫原品种 Histidine-tagged fusion protein corresponding to human AKAP1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human 抗体类型 Polyclonal Antibody 基因符号 - PRKA1
- AKAP1
- AKAP149
纯化方法 Antigen Affinity purified UniProt编号 - Q92667
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AKAP1 in HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 150 µL
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