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merck millipore,默克密理博,05-649,Anti-Chk2 Antibody, clone 7
产品名称：Anti-Chk2 Antibody, clone 7
产品型号：05-649
Anti-Chk2 Antibody, clone 7 is a mouse monoclonal antibody for detection of Chk2 also known as CHK2 checkpoint homolog (S. pombe), serine/threonine-protein kinase CHK2 & has been validated in WB, IP, More>>
merck millipore,默克密理博,05-649,Anti-Chk2 Antibody, clone 7
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-649 Replaces MABE491 品牌系列 Upstate
商名 - Upstate
描述 Anti-Chk2 Antibody, clone 7 Alternate Names - serine/threonine-protein kinase CHK2
- CHK2 (checkpoint, S.pombe) homolog
- CHK2 checkpoint homolog (S. pombe)
- checkpoint-like protein CHK2
- protein kinase CHK2
- protein kinase CHK2
背景信息 The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer. 产品信息 格式 Purified 控制 - Jurkat cell lysate, HeLa cell lysate
Included Positive Antigen Control: Catalog # 12-303, Jurkat Cell Lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Protein G purified mouse IgG1 buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C. 应用 应用 Anti-Chk2 Antibody, clone 7 is a mouse monoclonal antibody for detection of Chk2 also known as CHK2 checkpoint homolog (S. pombe), serine/threonine-protein kinase CHK2 & has been validated in WB, IP, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Chk2 from HeLa cells.
Immunocytochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect Chk2 in HeLa cells.生物信息 免疫原品种 Mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2 克隆 clone 7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Chk2 同种型 IgG1 品种反应性 MouseHuman Species Reactivity Note Human and mouse. Others not tested. Predicted to cross-react with rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001005735
Entrez基因汇总 In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. 基因符号 - Cds1
- CHEK2
- HuCds1
- CHK2
- LFS2
- RAD53
- bA444G7
- CDS1
- OTTHUMP00000028871
- PP1425
纯化方法 Protein G chromatography UniProt编号 - O96017
UniProt汇总 FUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
COFACTOR: Magnesium.
SIZE: 543 amino acids; 60915 Da
SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by western blot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells.
Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at from date of receipt. 包装信息 数量 200 µg
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