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merck millipore,默克密理博,AB1783,Anti-Glutamate Transporter Antibody, Glial
产品名称：Anti-Glutamate Transporter Antibody, Glial
产品型号：AB1783
Detect Glutamate Transporter using this Anti-Glutamate Transporter Antibody, Glial validated for use in IH(P), IF & WB.
merck millipore,默克密理博,AB1783,Anti-Glutamate Transporter Antibody, Glial
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Gp Serum Polyclonal Antibody 描述 产品目录编号 AB1783 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Glutamate Transporter Antibody, Glial Alternate Names - GLT-1, EAAT2
- GLT-1
背景信息 Glutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.
EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.产品信息 格式 Serum 控制 - Rat brain tissue
演示 Guinea pig antiserum containing no preservatives. 应用 应用 Detect Glutamate Transporter using this Anti-Glutamate Transporter Antibody, Glial validated for use in IH(P), IF & WB. 主要应用 - Western Blotting
应用说明 Immunohistochemistry:
1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde.
Immunohistochemistry:
1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody.
Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended.
Notes:
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the carboxy-terminus of rat GLT-1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Guinea Pig 特异性 Glial Glutamate Transporter GLT-1 (EAAT2). The antibody has been tested on central nervous system tissue. Preabsorption of the antiserum with the immunogen peptide (Catalog number AG391) completely abolishes the immunostaining. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004171.3
Entrez基因汇总 This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. 基因符号 - GLT1
- SLC1A2
- GLT-1
- EAAT2
纯化方法 Unpurified UniProt编号 - P43004
UniProt汇总 FUNCTION: SwissProt: P43004 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
SIZE: 574 amino acids; 62104 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Glycosylated.
SIMILARITY: SwissProt: P43004 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.产品使用声明 质量保证 Evaluated by Western Blot on mouse brain membrane lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 50 µL
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