merck millipore,默克密理博,71138,Rosetta-gami™ B(DE3)pLacI Competent Cells

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merck millipore,默克密理博,71138,Rosetta-gami™ B(DE3)pLacI Competent Cells - Novagen
产品名称：Rosetta-gami™ B(DE3)pLacI Competent Cells - Novagen
产品型号：71138
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance the expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.

merck millipore,默克密理博,71138,Rosetta-gami™ B(DE3)pLacI Competent Cells - Novagen

产品介绍

merck millipore,默克密理博,71138,Rosetta-gami™ B(DE3)pLacI Competent Cells - Novagen

描述
概述	This product has been discontinued. Expression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA. Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors. . Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors. In Rosetta-gami B(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
概述	Genotype: F^– ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLacIRARE (Cam^R, Kan^R, Tet^R)
产品目录编号	71138
品牌系列	Novagen®

产品信息
部件

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	≤ -70°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
布鲁克黑文许可证	This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

merck millipore,默克密理博,71138,Rosetta-gami™ B(DE3)pLacI Competent Cells - Novagen

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