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merck millipore,默克密理博,04-591,Anti-FAK Antibody, rabbit monoclonal, clone EP695Y
产品名称：Anti-FAK Antibody, rabbit monoclonal, clone EP695Y
产品型号：04-591
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-FAK Antibody, Rabbit, clone EP695Y is vali More>>
merck millipore,默克密理博,04-591,Anti-FAK Antibody, rabbit monoclonal, clone EP695Y
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-591 品牌系列 Upstate
商名 - Upstate
描述 Anti-FAK Antibody, rabbit monoclonal, clone EP695Y Alternate Names - Focal adhesion kinase
背景信息 Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). FAK expression is upregulated in various tumors (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). 产品信息 格式 Purified 演示 Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-FAK Antibody, Rabbit, clone EP695Y is validated for use in IP, WB, IC, IH(P) for the detection of FAK. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 WB: 1:1,000
IHC: 1:50-100 (paraffin-embedded human hepatocellular carcinoma)
ICC: 1:250-500 (Hela cells)
IP: 1:50生物信息 免疫原品种 A synthetic peptide corresponding to residues in human FAK. 克隆 EP695Y 宿主 Rabbit 特异性 Recognizes Human, Mouse, Rat FAK. 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, Rat (positive control: Hela cell lysate) 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_153831.2
- NM_005607.3
Entrez基因汇总 This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. 基因符号 - FAK1
- PTK2
- pp125FAK
- FAK
- FADK
纯化方法 Protein A purfied UniProt编号 - Q05397
UniProt汇总 FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20 °C. Stable for 12 months from date of receipt. 包装信息 数量 100 µL
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