merck millipore,默克密理博,HTS204RTA,Ready-to-Assay™ Y4 Neuropeptide Y Receptor Frozen Cells

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merck millipore,默克密理博,HTS204RTA,Ready-to-Assay™ Y4 Neuropeptide Y Receptor Frozen Cells
产品名称：Ready-to-Assay™ Y4 Neuropeptide Y Receptor Frozen Cells
产品型号：HTS204RTA
Human Y4 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS204RTA,Ready-to-Assay™ Y4 Neuropeptide Y Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS204RTA,Ready-to-Assay™ Y4 Neuropeptide Y Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	Y4

描述
产品目录编号	HTS204RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ Y₄ Neuropeptide Y Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptor family of G protein-coupled receptors. Five NPY receptors, Y1, Y2, Y4, Y5 and y6, have been defined at the molecular level, and each signals primarily through Gi/o. Binding of NPY family peptides to NPY receptors mediates a variety of physiological effects, including promotion of food intake, decreased anxiety, inhibition of neurotransmitter and hormone release, vasoconstriction, and gut motility. Y4 binds preferentially to PP, with significant binding to NPY and PYY (Michel et al., 1998). Y4 plays a role in control of reproduction, as Y4-null, ob/ob double knockout mice have increased fertility relative to ob/ob mice with functional Y4 (Sainsbury et al., 2002). In addition, mice lacking Y4 display resistance to weight gain induced by high fat diet, and mice lacking both Y2 and Y4 are resistant to the enhanced weight gain exhibited by Y1-null mice fed a high fat diet (Sainsbury et al., 2006). Millipore's cloned human Y4-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Y4 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at Y4 Receptor.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human Y4 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_005972
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	Y4
靶子系列	Neuropeptide Y
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.