merck millipore,默克密理博,S7750,TRAPeze ELISA Telomerase Detection Kit, strip well plates

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merck millipore,默克密理博,S7750,TRAPeze ELISA Telomerase Detection Kit, strip well plates
产品名称：TRAPeze ELISA Telomerase Detection Kit, strip well plates
产品型号：S7750
The TRAPeze ELISA Telomerase Detection Kit is a rapid, non-isotopic assay for detecting telomerase activity in cell or tissue extracts.

merck millipore,默克密理博,S7750,TRAPeze ELISA Telomerase Detection Kit, strip well plates

产品介绍

merck millipore,默克密理博,S7750,TRAPeze ELISA Telomerase Detection Kit, strip well plates

重要规格表

品种反应性	主要应用
Ma	RT-PCR

描述
产品目录编号	S7750
品牌系列	Chemicon®
商名	Chemicon TRAPeze
描述	TRAPeze® ELISA Telomerase Detection Kit, strip well plates
概述	The TRAPeze® ELISA Telomerase Detection Kit is a rapid, non-isotopic assay for detecting telomerase activity in cell or tissue extracts. The method combines a one-step TRAP assay (PCR) with direct, chromogenic detection of TRAP products (ELISA). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested. The kit uses coated strip-well pieces for ease of use and handling. Using this Manual The TRAPEZE® ELISA Telomerase Detection Kit provides reagents necessary to perform the Telomeric Repeat Amplification Protocol (TRAP) and ELISA assay for non-quantitative detection of telomerase activity in cells and tissues. The novice user is advised to read the entire manual prior to using the TRAPEZE® ELISA Kit, particularly Sec. III: Protocol. Supplemental protocols can be found in Sec. V. Appendix. Should additional questions arise, assistance can be obtained by contacting Chemicon Technical Support.
背景信息	Telomeres are specific structures found at the end of chromosomes in eukaryotes. In human chromosomes, the telomeres consist of thousands of copies of 6 base repeats (TTAGGG)(1-3). It has been suggested that telomeres protect chromosome ends since damaged chromosomes lacking telomeres undergo fusion, rearrangement and translocation (2). In somatic cells, telomere length is progressively shortened with each cell division both in vivo and in vitro (4-7) due to the inability of the DNA polymerase complex to synthesize the very 5' end of the lagging strand (8,9). Telomerase is a ribonucleoprotein that synthesizes and directs the telomeric repeats onto the 3' end of existing telomeres using its RNA component as a template (10-14). Telomerase activity has been shown to be specifically expressed in immortal cells, cancer and germ cells (15,16) where it compensates for telomere shortening during DNA replication and thus stabilizes telomere length (7,17). These observations have led to a hypothesis that telomere length may function as a "mitotic clock" to sense the number of cell divisions and eventually signal replicative senescence or programmed cell death when a critical telomere length is achieved. Therefore, expression of telomerase activity in cancer cells may be a necessary and essential step for tumor development and progression (16,18-20). The causal relationship between expression of telomerase and telomere length stabilization and the extension of the life span of the human cell has recently been reported (21). The development of a sensitive and efficient PCR-based telomerase activity detection method, TRAP (Telomeric Repeat Amplification Protocol)(15, 22), has made possible large scale surveys of telomerase activity in human cells and tissues (15, 23-29). To date, telomerase activity has been detected in over 85% of all tumors tested spanning more than 20 different types of cancers (30-31). The TRAPEZE® ELISA Kit provides the reagents necessary for performing the TRAP assay followed by ELISA detection of telomerase activity in cell/tissue samples.

产品信息
部件	* Caution - refer to Sec. II. Kit Components, Warning and Precautions. 1. CHAPS XL Lysis Buffer (10 mL) 2. 5X TRAPEZE® Reaction Mix (1 mL) • biotinylated TS primer • biotinylated RP primer •dA, dC, dG and dTTP •internal control primers •Tris buffer 3. PCR - Grade Water (10 mL) protease, DNase, and RNase-free; deionized 4. TSR8* (control template) (32 µL) 0.1 amole/µL TSR8 template 5. Control Cell Pellet Telomerase positive cells (106 cells) 6. Anti-DNP Antibody, HRP-conjugated (22µL) 7. Blocking/Dilution Buffer (45 mL) 8. 10X Washing Buffer (45 mL) 9. TMB Solution (10 mL) Tetramethylbenzidine (HRP substrate) 10. Stop Solution (10 mL) 11. Streptavidin-coated Microtiter Plate

产品信息

部件

* Caution - refer to Sec. II. Kit Components, Warning and Precautions.
1. CHAPS XL Lysis Buffer (10 mL)
2. 5X TRAPEZE® Reaction Mix (1 mL)
• biotinylated TS primer
• biotinylated RP primer
•dA, dC, dG and dTTP
•internal control primers
•Tris buffer
3. PCR - Grade Water (10 mL)
protease, DNase, and RNase-free;
deionized
4. TSR8* (control template) (32 µL)
0.1 amole/µL TSR8 template
5. Control Cell Pellet
Telomerase positive cells (106 cells)
6. Anti-DNP Antibody, HRP-conjugated
(22µL)
7. Blocking/Dilution Buffer (45 mL)
8. 10X Washing Buffer (45 mL)
9. TMB Solution (10 mL)
Tetramethylbenzidine (HRP substrate)
10. Stop Solution (10 mL)
11. Streptavidin-coated Microtiter Plate

应用
应用	The TRAPeze ELISA Telomerase Detection Kit is a rapid, non-isotopic assay for detecting telomerase activity in cell or tissue extracts.
主要应用	PCR

生物信息
品种反应性	Mammals

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Precautions 1. Because the TRAPEZE® ELISA Telomerase Detection Kit detects the activity of telomerase, a RNase sensitive ribonucleoprotein, and not merely the presence of the RNA or protein components of telomerase, the assay requires enzymatically active cell or tissue samples. Furthermore, due to the sensitivity of the TRAP assay, which can detect telomerase activity in a very small number of cells, a special laboratory setup and significant precautions are required to prevent PCR carry-over contamination and RNase contamination. These precautions are discussed in detail in Sec. V. Appendix, Laboratory Setup and Precautions and TRAP Station Setup. Before performing a TRAP assay, it is strongly recommended that you read the entire manual. At a minimum, read Sec. V. Appendix, Laboratory Setup and Precautions and Sec. III. Protocol, Experimental Design which describe all the controls that must be included in each analysis. 2. For Research Use Only. Not for use in diagnostic procedures.

储存和货运信息

存储条件

Precautions
1. Because the TRAPEZE® ELISA Telomerase Detection Kit detects the activity of telomerase, a RNase sensitive ribonucleoprotein, and not merely the presence of the RNA or protein components of telomerase, the assay requires enzymatically active cell or tissue samples. Furthermore, due to the sensitivity of the TRAP assay, which can detect telomerase activity in a very small number of cells, a special laboratory setup and significant precautions are required to prevent PCR carry-over contamination and RNase contamination. These precautions are discussed in detail in Sec. V. Appendix, Laboratory Setup and Precautions and TRAP Station Setup.
Before performing a TRAP assay, it is strongly recommended that you read the entire manual. At a minimum, read Sec. V. Appendix, Laboratory Setup and Precautions and Sec. III. Protocol, Experimental Design which describe all the controls that must be included in each analysis.
2. For Research Use Only. Not for use in diagnostic procedures.