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描述 概述 The ProteoExtract® Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures taking advantage of a novel titanium dioxide material. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis. 产品目录编号 539722 品牌系列 Calbiochem®
应用数据 
Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides (see below for concentrations). The samples were subsequently processed using the ProteoExtract® Phosphopeptide Enrichment TiO2 Kit as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode. (A) Unprocessed sample (B) Phosphopeptides recovered using the ProteoExtract® Phosphopeptide Enrichment TiO2 Kit. Spiked Phosphopeptides: 1. 50 nM Angiotensin ~6 pmol/125 µl; ~7 ng/125 µl 2. 67 nM Calcineurin Substrate ~8 pmol/125 µl; ~18 ng/125 µl 3. 140 nM α-Casein ~18 pmol/125 µl; ~400 ng/125 µl产品信息 检测方法 Mass Spectrometry 形式 100 Isolations 格式 Phosphopeptide enrichment 试剂盒包含 TiO₂ Phosphobind Resin, 10X TiO₂ Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO₂ Elution Buffer, Dihydroxybenzoic Acid, and a user protocol. 产品使用声明 预定用途 The ProteoExtract® Phosphopeptide Enrichment TiO₂ Kit is designed to enrich phosphorylated peptides from complex samples using a solid phase, highly selective, titanium oxide. The enriched phosphopeptide fraction is directly compatible with downstream analysis by MALDI and ESI mass spectrometry. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 +2°C to +8°C
存储条件 Upon arrival store the entire contents of the kit at 4°C. Following reconstitution of the Dihydroxybenzoic Acid refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage; reconstituted Dihydroxybenzoid Acid is stable for up to 6 weeks at 4°C or for up to 6 months at -20°C. 无需冷冻 No
补充信息 试剂盒包含 TiO₂ Phosphobind Resin, 10X TiO₂ Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO₂ Elution Buffer, Dihydroxybenzoic Acid, and a user protocol.
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