merck millipore,默克密理愽
  • merck millipore,默克密理博,69956,BLR(DE3)pLysS Competent Cells - Novagen

  • merck millipore,默克密理博,69956,BLR(DE3)pLysS Competent Cells - Novagen

    产品名称：BLR(DE3)pLysS Competent Cells - Novagen
    产品型号：69956
    BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.

    merck millipore,默克密理博,69956,BLR(DE3)pLysS Competent Cells - Novagen

  • 产品介绍
  • merck millipore,默克密理博,69956,BLR(DE3)pLysS Competent Cells - Novagen

    描述
    概述 BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).

    DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

    pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
    Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 pLysS (CamR, TetR)
    产品目录编号 69956
    品牌系列 Novagen®
    参考
    参考 1. A. Roca (U. of Wisconsin), personal
    communication.

    2. Studier, F. W. (1991) J. Mol. Biol. 219,
    37-44.
    产品信息
    部件
    保证高效 Guaranteed efficiency: > 2 x 10⁶ cfu/μg
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Multiple Toxicity Values, refer to MSDS
    储存 ≤ -70°C
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No
    布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

    merck millipore,默克密理博,69956,BLR(DE3)pLysS Competent Cells - Novagen

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